BACKGROUND Zygophyllum is an important medicinal plant, with notable properties such as resistance to salt, alkali, and drought, as well as tolerance of poor soils and shifting sand. However, the response mechanism of Zygophyllum spp. to abiotic stess were rarely studied. RESULTS Here, we aimed to explore the salt-tolerance genes of Zygophyllum plants by transcriptomic and metabolic approaches. We chose Z. brachypterum, Z. obliquum and Z. fabago to screen for salt tolerant and sensitive species. Cytological observation showed that both the stem and leaf of Z. brachypterum were significantly thicker than those of Z. fabago. Then, we treated these three species with different concentrations of NaCl, and found that Z. brachypterum exhibited the highest salt tolerance (ST), while Z. fabago was the most sensitive to salt (SS). With the increase of salt concentration, the CAT, SOD and POD activity, as well as proline and chlorophyll content in SS decreased significantly more than in ST. After salt treatment, the proportion of open stomata in ST decreased significantly more than in SS, although there was no significant difference in stomatal number between the two species. Transcriptomic analysis identified a total of 11 overlapping differentially expressed genes (DEGs) in the leaves and roots of the ST and SS species after salt stress. Two branched-chain-amino-acid aminotransferase (BCAT) genes among the 11 DEGs, which were significantly enriched in pantothenate and CoA biosynthesis, as well as the valine, leucine and isoleucine biosynthesis pathways, were confirmed to be significantly induced by salt stress through qRT-PCR. Furthermore, overlapping differentially abundant metabolites showed that the pantothenate and CoA biosynthesis pathways were significantly enriched after salt stress, which was consistent with the KEGG pathways enriched according to transcriptomics. CONCLUSIONS In our study, transcriptomic and metabolomic analysis revealed that BCAT genes may affect the pantothenate and CoA biosynthesis pathway to regulate the salt tolerance of Zygophyllum species, which may constitute a newly identified signaling pathway through which plants respond to salt stress.

中文翻译：

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转录组和代谢组学分析揭示了CoA在霸王藻耐盐性中的作用。

背景技术霸王是重要的药用植物，具有显着的特性，例如对盐，碱和干旱的抵抗力，以及对贫瘠土壤和流沙的耐受性。然而，霸王的响应机制。到非生物类植物的研究很少。结果在这里，我们旨在通过转录和代谢方法探索霸王植物的耐盐基因。我们选择了Z. brachypterum，Z。obliquum和Z. fabago来筛选耐盐和敏感物种。细胞学观察表明，短臂Z.的茎和叶均明显比fabago厚。然后，我们用不同的NaCl浓度处理了这三个物种，结果发现短臂梭菌表现出最高的耐盐性（ST），而短尾梭菌对盐最敏感（SS）。随着盐浓度的增加，SS中的CAT，SOD和POD活性以及脯氨酸和叶绿素含量的下降明显大于ST。盐处理后，尽管两种物种之间的气孔数量没有显着差异，但ST中开放气孔的比例比SS显着下降更多。转录组分析鉴定出盐胁迫后，ST和SS物种的叶和根中共有11个重叠的差异表达基因（DEG）。在11个DEG中有两个支链氨基酸氨基酸转移酶（BCAT）基因，它们在泛酸和CoA生物合成以及缬氨酸，亮氨酸和异亮氨酸的生物合成途径中显着富集，被证实是通过盐胁迫显着诱导的。定量RT-PCR。此外，重叠的差异丰富的代谢产物表明，泛酸和CoA的生物合成途径在盐胁迫后显着富集，这与根据转录组学富集的KEGG途径一致。结论在我们的研究中，转录组和代谢组学分析表明BCAT基因可能影响泛酸和CoA生物合成途径来调节霸王藻的耐盐性，这可能构成了植物识别盐胁迫的新信号通路。