High resolution, label-free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell.,Journal of Biophotonics

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High resolution, label-free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-01-13 , DOI: 10.1002/jbio.201960108
Arunima Sharma ₁ , Noreen Ishak _{1,

2} , Teoh Swee-Hin _{1,

3} , Manojit Pramanik ₁

Affiliation

Chicken embryos have been proven to be an attractive vertebrate model for biomedical research. They have helped in making significant contributions for advancements in various fields like developmental biology, cancer research and cardiovascular studies. However, a non‐invasive, label‐free method of imaging live chicken embryo at high resolution still needs to be developed and optimized. In this work, we have shown the potential of photoacoustic tomography (PAT) for imaging live chicken embryos cultured in bioengineered eggshells. Laser pulses at wavelengths of 532 and 740 nm were used for attaining cross‐sectional images of chicken embryos at different developmental stages. Cross‐sections along different depths were imaged to gain knowledge of the relative depth of different vessels and organs. Due to high optical absorption of vasculature and embryonic eye, images with good optical contrast could be acquired using this method. We have thus reported a label‐free method of performing cross‐sectional imaging of chicken embryos at high resolution demonstrating the capacity of PAT as a promising tool for avian embryo imaging.

中文翻译：

在生物工程蛋壳中发育的活鸡胚胎的高分辨率，无标签光声成像。

鸡胚已被证明是用于生物医学研究的有吸引力的脊椎动物模型。他们为发展生物学，癌症研究和心血管研究等各个领域的进步做出了重大贡献。但是，仍需要开发和优化一种高分辨率的活鸡胚成像的无创，无标签方法。在这项工作中，我们显示了光声层析成像（PAT）的潜力，可以对生物工程蛋壳中培养的活鸡胚胎进行成像。使用532和740 nm波长的激光脉冲获得不同发育阶段鸡胚的横截面图像。对不同深度的横截面进行成像，以了解不同血管和器官的相对深度。由于脉管系统和胚胎眼的高光吸收，使用这种方法可以获得具有良好光学对比度的图像。因此，我们已经报道了一种无标记方法，可以对鸡胚进行高分辨率的横截面成像，证明了PAT作为禽胚胎成像的有前途的工具的能力。

更新日期：2020-01-13

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