Turbidimetric analysis of growth kinetics of bacteria in the laboratory environment using smartphone.,Journal of Biophotonics

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Turbidimetric analysis of growth kinetics of bacteria in the laboratory environment using smartphone.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-01-16 , DOI: 10.1002/jbio.201960159
Diganta Hatiboruah ₁ , Damayanti Y Devi ₂ , Nima D Namsa ₂ , Pabitra Nath ₁

Affiliation

For different microbiological and pathological studies, it is often required to monitor the growth of bacteria in a cultured medium in the laboratory environment. UV‐VIS spectrophotometer is commonly used to estimate the growth of bacterial cell population by measuring the absorbance at 600 nm over a period of time. Colony‐forming unit (CFU) is another approach, which has been routinely performed to estimate the live bacterial cells on semisolid agar plates. Herein, we demonstrate an alternative yet highly reliable sensing platform on a smartphone using which growth kinetics of different bacteria can be reliably monitored. The performance of the proposed smartphone sensor has been compared with the data obtained from OD600 and CFU analysis. A good correlation of bacterial growth rates enumerated based on the proposed smartphone sensor, bench‐top spectrophotometer and CFU analysis have been observed under the experimental conditions.

中文翻译：

使用智能手机对实验室环境中细菌的生长动力学进行比浊分析。

对于不同的微生物学和病理学研究，通常需要在实验室环境中监测培养基中细菌的生长。UV-VIS分光光度计通常用于通过测量一段时间内600 nm处的吸光度来估计细菌细胞群的生长。集落形成单位（CFU）是另一种方法，已常规执行以估计半固体琼脂平板上的活细菌细胞。在本文中，我们演示了智能手机上的一种替代性但高度可靠的传感平台，使用该平台可以可靠地监视不同细菌的生长动力学。拟议的智能手机传感器的性能已与从OD600和CFU分析获得的数据进行了比较。根据建议的智能手机传感器，可以得出细菌生长速率的良好相关性，

更新日期：2020-01-16

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