Abstract The current consumer demand for healthier diets, the growing interest in the search for new sources of protein, and the desire to reduce the negative effects on the environment have increased interest in the study of insect proteins. The present study focused on the technofunctional characteristics (interfacial and foaming properties) and the in-vitro antioxidant activity of a protein concentrate obtained from silkworm (Bombyx mori) pupae (SPC). The isoelectric point of the SPC was close to pH 4.0–5.0 as determined by protein solubility and z potential analysis. Given that the SPC had solubilities of ~50% and z potentials of ~20 mV at pH 2.0 and 8.0, it was decided to further study SPC properties at these pH values. The supernatant obtained after adjustment of SPC to pH 8.0 showed higher (p k D ∗ = 69.2 ± 0.4 Vs. 29.5 ± 0.9 m N / m · s − 1 / 2 at pH 2.0). However, lower kinetic values at pH 2.0 increased the elastic behaviour of the viscoelastic interfacial film formed (E's ⁓ 30 mN/m at pH 2.0 V. E's ⁓ 20 mN/m at pH 8.0), which can be related with the higher protein sizes found at pH 2.0. These rearrangements of the SPC components appeared to increase its foaming capacity, whereas the foaming capacity of SPC adjusted to pH 8.0 was minimal.

中文翻译：

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家蚕 (Bombyx mori) 蛹蛋白浓缩物的界面/发泡特性和抗氧化活性

摘要 当前消费者对更健康饮食的需求、对寻找新蛋白质来源的兴趣日益增长以及减少对环境的负面影响的愿望增加了对昆虫蛋白质研究的兴趣。本研究侧重于从家蚕 (Bombyx mori) 蛹 (SPC) 中获得的浓缩蛋白的技术功能特性（界面和发泡特性）和体外抗氧化活性。通过蛋白质溶解度和 z 电位分析确定 SPC 的等电点接近 pH 值 4.0-5.0。鉴于 SPC 在 pH 2.0 和 8.0 下具有 ~50% 的溶解度和 ~20 mV 的 z 电位，决定进一步研究这些 pH 值下的 SPC 特性。将 SPC 调节至 pH 8.0 后获得的上清液显示更高（pk D * = 69.2 ± 0.4 Vs. 29.5 ± 0。9 m N / m · s − 1 / 2，pH 2.0)。然而，在 pH 2.0 时较低的动力学值增加了形成的粘弹性界面膜的弹性行为（E's ⁓ 30 mN/m at pH 2.0 V. E's ⁓ 20 mN/m at pH 8.0），这可能与较高的蛋白质尺寸有关在 pH 2.0 时发现。SPC 组分的这些重排似乎增加了其发泡能力，而调节至 pH 8.0 的 SPC 的发泡能力很小。