当前位置: X-MOL 学术Talanta › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Correlated quantification using microbiological and electrochemical assays for roxithromycin determination in biological and pharmaceutical samples.
Talanta ( IF 5.6 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.talanta.2019.120703
Abdelghani Mahmoudi 1 , Mihaela Tertiş 2 , Laura-Mihaela Simon 3 , Ann Van Schepdael 4 , Silvia De Francia 5 , Lia-Monica Junie 3 , Robert Săndulescu 2
Affiliation  

Microbiological and electrochemical assays, applying the cylinder-plate and differential pulse voltammetry as techniques, are reported for the quantitative determination of roxithromycin in serum and solid pharmaceutical form. The microbiological assay is based upon the inhibitory effect of this drug on the strain Bacillus subtilis ATCC 9372 used as the test microorganism. Linearity of the calibration curve was observed over the concentration range of 8.37-83.70 μg mL-1, with relative standard deviation values less than 5.0%. The electrochemical behavior of roxithromycin was studied at a graphite screen-printed electrode modified with graphene by using cyclic voltammetry and differential pulse voltammetry. The current value of the oxidative peak obtained for roxithromycin at 0.65 V vs. Ag/AgCl in 0.03 mol L-1 phosphate buffer solution (pH 7.0) with a scan rate of 0.1 V-1 is a linear function of the concentration in a range of 4.19-83.70 μg mL-1 (5-100 μmol L-1). A comparative study was carried out and both methods were applied for the determination of roxithromycin in solid dosage forms and spiked serum. The bioassay results of human serum samples were in accordance with the electrochemical ones (R2 = 0.988, P < 0.001), and the Bland-Altman method also showed good agreement between the values obtained by both procedures. Moreover, the statistical comparison indicated that there was no significant difference between the proposed techniques regarding both accuracy and precision.

中文翻译:

使用微生物和电化学测定法进行相关定量,以测定生物和药物样品中的罗红霉素。

据报道,采用圆筒板和差分脉冲伏安法作为技术的微生物学和电化学测定法可定量测定血清和固体药物形式的罗红霉素。微生物测定是基于该药物对用作测试微生物的枯草芽孢杆菌ATCC 9372的抑制作用。在8.37-83.70μgmL-1的浓度范围内观察到校准曲线的线性,相对标准偏差值小于5.0%。利用循环伏安法和微分脉冲伏安法研究了罗红霉素在石墨烯修饰的石墨丝网印刷电极上的电化学行为。在0.03 mol L-1磷酸盐缓冲溶液(pH 7.)中,罗红霉素在0.65 V相对于Ag / AgCl的氧化峰的电流值。扫描速率为0.1 V-1的0)是浓度在4.19-83.70μgmL-1(5-100μmolL-1)范围内的线性函数。进行了比较研究,两种方法均用于测定固体剂型和加标血清中的罗红霉素。人血清样品的生物测定结果与电化学测定结果一致(R2 = 0.988,P <0.001),并且Bland-Altman方法在两种方法获得的值之间也显示出良好的一致性。此外,统计比较表明,在准确性和精度方面,所提出的技术之间没有显着差异。进行了比较研究,两种方法均用于测定固体剂型和加标血清中的罗红霉素。人血清样品的生物测定结果与电化学测定结果一致(R2 = 0.988,P <0.001),并且Bland-Altman方法在两种方法获得的值之间也显示出良好的一致性。此外,统计比较表明,在准确性和精度方面,所提出的技术之间没有显着差异。进行了比较研究,两种方法均用于测定固体剂型和加标血清中的罗红霉素。人血清样品的生物测定结果与电化学测定结果一致(R2 = 0.988,P <0.001),并且Bland-Altman方法在两种方法获得的值之间也显示出良好的一致性。此外,统计比较表明,在准确性和精度方面,所提出的技术之间没有显着差异。
更新日期:2020-01-07
down
wechat
bug