Glutathione (GSH) plays an important role in the normal physiological activities of human body. It is significant to establish a simple and rapid analysis method to detect accurately GSH. In this work, a “turn-on” fluorescence platform based on conjugated polymer nanoparticles (CPNs) and MnO₂ nanosheets was constructed for quantitative detection of GSH. The polymer of PBEC (poly [3-{2,5-bis (2-ethyl-hexyloxy)}-phenyl]-vinyl]-9-ethyl-carbazole]) designed by our group, was co-precipitated to prepare the conjugated polymer nanoparticles (CPNs_PBEC). Combined with MnO₂ nanosheets through van der waals force, a novel CPNs_PBEC-MnO₂ nano-platform was established based on the fluorescence resonance energy transfer (FRET). The fluorescence of CPNs_PBEC will be recovered in the presence of GSH because the MnO₂ nanosheets were reduced to Mn²⁺, so the detection of GSH was realized. A limit of detection (LOD) of CPNs_PBEC-MnO₂ nano-platform is 20.4 nM. Furthermore, this nano-platform with high selectivity and sensitivity was applied successfully to the determination of GSH in mouse serum and plasma. All of these indicated that the nano-platform could be used to effectively monitor GSH and expected to achieve early diagnosis of GSH-related diseases.

谷胱甘肽（GSH）在人体的正常生理活动中起着重要作用。建立一种简单，快速的分析方法以准确检测GSH具有重要意义。在这项工作中，构建了一个基于共轭聚合物纳米粒子（CPN）和MnO ₂纳米片的“开启”荧光平台，用于定量检测GSH。将由我们小组设计的PBEC（聚[3- {2,5-双（2-乙基-己氧基）}-苯基]-乙烯基] -9-乙基咔唑]聚合物共沉淀制备共轭聚合物纳米粒子（_{CPNs PBEC}）。通过范德华力与MnO ₂纳米片结合，形成新型_CPNs PBEC -MnO ₂基于荧光共振能量转移（FRET）建立了纳米平台。由于MnO ₂纳米片被还原为Mn ²⁺，因此在GSH存在下_{CPNs PBEC}的荧光得以恢复，从而实现了GSH的检测。_CPNs PBEC -MnO ₂纳米平台的检出限（LOD）为20.4 nM。此外，这种具有高选择性和灵敏度的纳米平台已成功应用于小鼠血清和血浆中GSH的测定。所有这些表明，纳米平台可用于有效监测GSH，并有望实现GSH相关疾病的早期诊断。