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Leakage and Rupture of Lipid Membranes by Charged Polymers and Nanoparticles.
Langmuir ( IF 3.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.langmuir.9b03301 Yibo Liu 1 , Juewen Liu 1
Langmuir ( IF 3.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.langmuir.9b03301 Yibo Liu 1 , Juewen Liu 1
Affiliation
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Understanding and controlling the interactions between lipid membranes and nanomaterials are important for drug delivery, toxicity studies, and sensing. In the literature, the perception is that cationic nanomaterials can damage lipid membranes, although some reports suggest the opposite. In this work, instead of using different materials for testing the effect of charge, we used the same material and adjusted the pH. A total of three types of liposomes including zwitterionic phosphocholine (PC) and negatively charged phosphoserine (PS) with saturated and unsaturated tails were tested with three types of metal oxide nanoparticles and two types of cationic polymers. A calcein leakage assay was used to probe membrane leakage. We found that cationic polymers had very little advantage for leaking PC liposomes. On the other hand, the PS liposomes were leaked by TiO2 nanoparticles regardless of their charge tuned by pH. ZnO with a high pKa value was studied in detail, and it only leaked the 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes at low pH when ZnO was positively charged, but leakage was inhibited by adding NaCl to weaken electrostatic attraction and by capping ZnO. In addition, dissolution of adsorbed ZnO also caused leakage, suggesting that adsorption and desorption induced reversible lipid phase transitions. Overall, the interaction strength was a key factor for leakage. Leakage does not necessarily mean membrane damage, and cryogenic transmission electron microscopy was used to study membrane integrity. Previously observed cationic polymer/nanoparticle-induced damages in supported membranes could be due to electrostatic attraction between the polymers and the underlying negatively charged supporting surface.
中文翻译：
带电聚合物和纳米颗粒对脂质膜的泄漏和破裂。
理解和控制脂质膜与纳米材料之间的相互作用对于药物输送，毒性研究和传感至关重要。在文献中，人们认为阳离子纳米材料会损害脂质膜，尽管一些报道表明相反。在这项工作中，我们使用相同的材料并调节了pH值，而不是使用不同的材料来测试电荷效应。用三种类型的金属氧化物纳米颗粒和两种类型的阳离子聚合物测试了总共三种类型的脂质体，包括两性离子磷酸胆碱（PC）和带饱和和不饱和尾巴的带负电荷的磷酸丝氨酸（PS）。钙黄绿素泄漏测定法用于探测膜泄漏。我们发现阳离子聚合物在泄漏PC脂质体方面几乎没有优势。另一方面，TiO2纳米颗粒泄漏了PS脂质体，无论它们的电荷是如何通过pH调节的。详细研究了高pKa值的ZnO，当ZnO带正电时，它仅在低pH下泄漏1,2-二醇基-sn-甘油3-磷酸胆碱脂质体，但通过添加NaCl抑制泄漏，以减弱静电吸引力并通过覆盖ZnO。此外，吸附的ZnO的溶解也会引起渗漏，表明吸附和解吸会引起可逆的脂质相变。总体而言，相互作用强度是渗漏的关键因素。泄漏并不一定意味着膜损坏，因此使用了低温透射电子显微镜来研究膜的完整性。
更新日期：2020-01-17
中文翻译：
带电聚合物和纳米颗粒对脂质膜的泄漏和破裂。
理解和控制脂质膜与纳米材料之间的相互作用对于药物输送，毒性研究和传感至关重要。在文献中，人们认为阳离子纳米材料会损害脂质膜，尽管一些报道表明相反。在这项工作中，我们使用相同的材料并调节了pH值，而不是使用不同的材料来测试电荷效应。用三种类型的金属氧化物纳米颗粒和两种类型的阳离子聚合物测试了总共三种类型的脂质体，包括两性离子磷酸胆碱（PC）和带饱和和不饱和尾巴的带负电荷的磷酸丝氨酸（PS）。钙黄绿素泄漏测定法用于探测膜泄漏。我们发现阳离子聚合物在泄漏PC脂质体方面几乎没有优势。另一方面，TiO2纳米颗粒泄漏了PS脂质体，无论它们的电荷是如何通过pH调节的。详细研究了高pKa值的ZnO，当ZnO带正电时，它仅在低pH下泄漏1,2-二醇基-sn-甘油3-磷酸胆碱脂质体，但通过添加NaCl抑制泄漏，以减弱静电吸引力并通过覆盖ZnO。此外，吸附的ZnO的溶解也会引起渗漏，表明吸附和解吸会引起可逆的脂质相变。总体而言，相互作用强度是渗漏的关键因素。泄漏并不一定意味着膜损坏，因此使用了低温透射电子显微镜来研究膜的完整性。
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