We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimentally ruled out that oscillations originate in extra- or intracellular K+ fluxes and conclude that these oscillations arise from fluctuations in free and adsorbed states of K+ in the cell interior. Oscillations in K+ showed a strong dependence on ATP and the organization of the cell cytoskeleton. Our results challenge the widely held view that intracellular K+ predominantly exists in a free state. They can, however, be productively understood in terms of Gilbert Ling's Association-Induction hypothesis.

中文翻译：

---

糖酵解振荡和细胞内K +浓度在酿酒酵母中紧密耦合。

我们使用荧光光谱法和显微镜方法测量了酵母细胞中糖酵解振荡时细胞内钾离子浓度的时间振荡。这些振荡显示出与糖酵解代谢产物（NADH，ATP）相同的周期，表明它们之间存在强耦合。我们通过实验排除了振荡起源于细胞外或细胞内K +通量，并得出结论，这些振荡是由于细胞内部K +的自由态和吸附态的波动引起的。K +的振荡显示出对ATP和细胞骨架结构的强烈依赖性。我们的结果挑战了普遍持有的观点，即细胞内K +主要以游离状态存在。但是，可以根据吉尔伯特·林（Gilbert Ling）的关联归纳假设来有效地理解它们。