当前位置：
X-MOL 学术
›
Phytochemistry
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Salinity effects on physiological and phytochemical characteristics and gene expression of two Glycyrrhiza glabra L. populations
Phytochemistry ( IF 3.2 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.phytochem.2019.112236 Assieh Behdad 1 , Sasan Mohsenzadeh 1 , Majid Azizi 2 , Nasrin Moshtaghi 3
Phytochemistry ( IF 3.2 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.phytochem.2019.112236 Assieh Behdad 1 , Sasan Mohsenzadeh 1 , Majid Azizi 2 , Nasrin Moshtaghi 3
Affiliation
|
Glycyrrhiza glabra (licorice) is a medicinal plant with valuable specialised metabolites such as triterpene sweetener glycyrrhizin. Salinity stress is the main environmental stress limiting plant growth and development. The effects of six levels of NaCl (0, 100, 200, 400, 600, and 800 mM) on growth, osmolyte content, oxidative stress markers, antioxidant enzyme activities, K+/Na+ ratio, glycyrrhizin content, and gene expression of glycyrrhizin biosynthesis (bAS, CYP88D6, and CYP72A154) were investigated in licorice rhizomes of two populations. The results showed that the salt stress progressively reduced the growth parameters and increased the proline concentrations in the rhizomes. K+/Na+ ratio showed a significant decrease under salinity as compared to the controls. Salt stress resulted in oxidative stress on the rhizomes, as indicated by increased lipid peroxidation and hydrogen peroxide concentrations and elevated the activities of antioxidant enzymes (i.e., ascorbate peroxidase and superoxide dismutase). The glycyrrhizin content increased only under 100 and 200 mM NaCl treatments. The same trend was observed in the expression of bAS, CYP88D6, and CYP72A154 genes in Fars population. Fars population was found to have more glycyrrhizin content than Khorasan population. But, growth, glycyrrhizin content, and biosynthesis genes of glycyrrhizin showed more reduction in Khorasan population as compared to those of Fars population. The results indicate that the application of 100 mM NaCl up-regulated the expression of key genes involved in the biosynthesis of triterpenoid saponins and directly enhanced the production of glycyrrhizin. Accordingly, G. glabra can be introduced as a halophyte plant.
中文翻译：
盐度对两个光果甘草种群生理、植物化学特性及基因表达的影响
光果甘草（甘草）是一种药用植物，具有宝贵的特殊代谢物，如三萜甜味剂甘草甜素。盐分胁迫是限制植物生长发育的主要环境胁迫。六种浓度的 NaCl（0、100、200、400、600 和 800 mM）对生长、渗透物含量、氧化应激标志物、抗氧化酶活性、K+/Na+ 比、甘草甜素含量和甘草甜素生物合成基因表达的影响（bAS、CYP88D6 和 CYP72A154）在两个群体的甘草根茎中进行了研究。结果表明，盐胁迫逐渐降低了根茎中的生长参数并增加了脯氨酸浓度。与对照相比，K+/Na+ 比值在盐度下显着降低。盐胁迫导致根茎受到氧化胁迫，如脂质过氧化和过氧化氢浓度增加以及抗氧化酶（即抗坏血酸过氧化物酶和超氧化物歧化酶）活性升高所表明的。甘草甜素含量仅在 100 和 200 mM NaCl 处理下增加。Fars人群中bAS、CYP88D6和CYP72A154基因的表达也有相同趋势。发现 Fars 人群的甘草甜素含量高于 Khorasan 人群。但是，与 Fars 种群相比，Khorasan 种群的生长、甘草甜素含量和甘草甜素的生物合成基因显示出更多的减少。结果表明，100 mM NaCl的应用上调了参与三萜皂苷生物合成的关键基因的表达，并直接提高了甘草甜素的产量。因此，G.
更新日期：2020-03-01
中文翻译：
盐度对两个光果甘草种群生理、植物化学特性及基因表达的影响
光果甘草（甘草）是一种药用植物，具有宝贵的特殊代谢物，如三萜甜味剂甘草甜素。盐分胁迫是限制植物生长发育的主要环境胁迫。六种浓度的 NaCl（0、100、200、400、600 和 800 mM）对生长、渗透物含量、氧化应激标志物、抗氧化酶活性、K+/Na+ 比、甘草甜素含量和甘草甜素生物合成基因表达的影响（bAS、CYP88D6 和 CYP72A154）在两个群体的甘草根茎中进行了研究。结果表明，盐胁迫逐渐降低了根茎中的生长参数并增加了脯氨酸浓度。与对照相比，K+/Na+ 比值在盐度下显着降低。盐胁迫导致根茎受到氧化胁迫，如脂质过氧化和过氧化氢浓度增加以及抗氧化酶（即抗坏血酸过氧化物酶和超氧化物歧化酶）活性升高所表明的。甘草甜素含量仅在 100 和 200 mM NaCl 处理下增加。Fars人群中bAS、CYP88D6和CYP72A154基因的表达也有相同趋势。发现 Fars 人群的甘草甜素含量高于 Khorasan 人群。但是，与 Fars 种群相比，Khorasan 种群的生长、甘草甜素含量和甘草甜素的生物合成基因显示出更多的减少。结果表明，100 mM NaCl的应用上调了参与三萜皂苷生物合成的关键基因的表达，并直接提高了甘草甜素的产量。因此，G.
京公网安备 11010802027423号