Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.

植物乳杆菌由于其在免疫调节和维持肠道通透性中的作用而被鉴定为益生菌。在这里，我们研究了L的抗结肠炎作用及其机理。车前草CBT LP3（LP3）。这种体内这项研究是使用葡聚糖硫酸钠（DSS）诱导小鼠结肠炎进行的。将小鼠随机分为三组：提供正常饮用水的对照组，DSS治疗组，随后口服赋形剂，和DSS治疗组，每天服用LP3，持续7天。使用流式细胞术分析从腹膜腔细胞和脾细胞收获的巨噬细胞和T细胞亚群。使用定量逆转录酶聚合酶链反应测量基因表达和细胞因子概况。与对照相比，LP3的施用显着减弱了疾病活性和组织病理学。LP3具有抗炎作用，脾细胞中调节性T细胞和2型辅助性T细胞的诱导作用增强，杯状细胞恢复，同时抑制促炎性细胞因子表达。这些发现表明植物乳杆菌CBT LP3可用作有效的免疫调节剂，对IBD治疗有重要意义。