Copper (Cu) deficiency plays an important role in the development of cardiovascular disorders. Resveratrol (RSV) possesses pleiotropic cardiovascular benefits; however, the mechanism(s) by which RSV exerts protective effects are not completely understood. Male Wistar rats at 6 weeks of age were fed for 8 weeks with a Cu deficient diet (no added Cu, Cu = 0). In addition, Cu deficient rats were supplemented with RSV (500 mg/kg of diet, n = 9). Blood and intestinal samples were taken for further analysis together with internal organs and thoracic arteries. RSV supplementation resulted in elevated blood plasma levels of Cu (x2.1) and Zn (x1.1), in an increased activity of superoxide dismutase (SOD, x1.5) and ferric reducing antioxidant power (FRAP, x1.2). Meanwhile, markers of lipid peroxidation expressed as malondialdehyde (MDA, x1.5) and lipid hydroperoxides (LOOH, x1.1) were also increased in a significant way. Food intake, body weight, blood glucose, catalase, ceruloplasmin, lipid profile and intestinal samples were not modified. RSV enhanced the vasoconstriction of isolated thoracic arteries to noradrenaline (x1.4), potentiated the vasodilation to acetylcholine (ACh, x1.4) and increased the sensitivity to sodium nitroprusside (SNP). In addition, preincubation with the cyclooxygenase (COX)-inhibitor, indomethacin, potentiated the ACh-induced vasodilation, which was more pronounced in animals not supplemented with RSV. The KATP channel opener, pinacidil, induced a similar response in both studied groups. In conclusion, this study demonstrates that RSV supplementation influences oxidative stress and the antioxidant status, which may modify the vascular response in Cu deficiency.

中文翻译：

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白藜芦醇调节铜缺乏的Wistar大鼠的血浆铜和锌水平，抗氧化剂状态和胸动脉的血管反应。

铜缺乏在心血管疾病的发展中起重要作用。白藜芦醇（RSV）具有多效性心血管益处；但是，RSV发挥保护作用的机制尚不完全清楚。用缺铜饮食（不添加铜，铜= 0）喂养6周龄的雄性Wistar大鼠，持续8周。另外，对铜缺乏的大鼠补充RSV（500 mg / kg饮食，n = 9）。抽取血液和肠道样本以及内部器官和胸动脉进行进一步分析。补充RSV会导致血浆Cu（x2.1）和Zn（x1.1）升高，超氧化物歧化酶（SOD，x1.5）活性增强，三氧化二铁的抗氧化能力降低（FRAP，x1.2）。同时，脂质过氧化的标志物表示为丙二醛（MDA，x1。5）和脂质氢过氧化物（LOOH，x1.1）也以显着方式增加。食物摄入量，体重，血糖，过氧化氢酶，铜蓝蛋白，血脂和肠道样本均未改变。RSV增强了离体胸动脉对去甲肾上腺素（x1.4）的血管收缩，增强了对乙酰胆碱（ACh，x1.4）的血管舒张作用，并增加了对硝普钠（SNP）的敏感性。此外，与环氧合酶（COX）抑制剂吲哚美辛的预孵育增强了ACh诱导的血管舒张，这在未添加RSV的动物中更为明显。在两个研究组中，KATP通道开放剂吡那地尔诱导了相似的反应。总之，这项研究表明补充RSV会影响氧化应激和抗氧化剂状态，