Besides its popular refreshing value, tea is globally consumed as a potential health beverage. Health benefit traits primarily rely on some regulatory networks of different metabolic pathways. In tea, the trait‐specific simple sequence repeat (SSR) markers are still insufficient, which could be used for marker‐assisted breeding technique. MicroRNAs are endogenous, non‐coding, short RNAs directly involved in regulating gene expressions at the post‐transcriptional level. It has been found that differences in miRNA precursor gene sequences interfere their structure formation and obstruct subsequent function. SSR motifs within the putative miRNA precursor genes have been identified followed by experimental validation of their existence and variation exclusively in tea. Simultaneously, diverse commercial accessions of Indian tea were undergone rigorous phenotyping during three plucking seasons to assess 20 chemotypic traits by high‐throughput techniques. Six miRNA‐SSR marker‐generated bands consisted the putative trait‐associated loci. Newly developed microRNAs (mi408, miR5021a and miR2863) precursor‐derived SSR markers, miSSR‐03, miSSR‐11 and miSSR‐14, executed convincing results in distinguishing tea cultivars with contrasting health benefit traits. This is in accordance with the earlier reports where mi408, miR5021a and miR2863 have been found to be involved in cellular pathways conferring tolerance to oxidative stress. Consequently, these markers can be used further as suitable choice to select promising tea genotypes with sound antioxidant quality.

中文翻译：

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miRNA前体来源的具有不同健康益处性状的茶树（茶树）的SSR标记介导的基因分型

除了其令人耳目一新的提神价值外，茶还作为一种潜在的健康饮料在全球范围内消费。健康益处特征主要依赖于不同代谢途径的某些调节网络。在茶中，性状特异的简单序列重复（SSR）标记仍然不足，可用于标记辅助育种技术。MicroRNA是内源性非编码短RNA，直接参与转录后水平的基因表达调控。已经发现miRNA前体基因序列的差异会干扰其结构形成并阻碍随后的功能。已经鉴定出推定的miRNA前体基因中的SSR模体，然后通过实验验证了它们在茶中的存在和变异。同时，在三个采摘季节，对印度茶的各种商业登录品进行了严格的表型分析，以通过高通量技术评估20种化学型特征。六个miRNA‐SSR标记产生的条带组成了假定的性状相关基因座。最新开发的microRNA（mi408，miR5021a和miR2863）前体来源的SSR标记物miSSR-03，miSSR-11和miSSR-14具有令人信服的结果，可区分具有不同健康益处特征的茶树品种。这与较早的报道一致，其中已发现mi408，miR5021a和miR2863参与赋予氧化应激耐受性的细胞途径。因此，这些标记物可以进一步用作选择具有良好抗氧化剂质量的有前途的茶基因型的合适选择。