Biomaterial‐associated thrombus formation and bacterial infection remain major challenges for blood‐contacting devices. For decades, titanium‐based implants have been largely used for different medical applications. However, titanium can neither suppress blood coagulation, nor prevent bacterial infections. To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed. The surfaces were characterized by scanning electron microscopy (SEM), X‐ray photoelectron spectroscopy (XPS), and water contact angle measurements. To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed. The antibacterial activity was investigated against Gram‐negative P. aeruginosa and Gram‐positive S. aureus. Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM. The anti‐thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation. Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation. The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.

中文翻译：

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使用tanfloc/肝素聚电解质多层膜增强二氧化钛纳米管的血液相容性和抗菌活性。

生物材料相关的血栓形成和细菌感染仍然是血液接触装置的主要挑战。几十年来，钛基植入物已广泛用于不同的医疗应用。然而，钛既不能抑制血液凝固，也不能防止细菌感染。为了应对这些挑战，开发了二氧化钛纳米管阵列表面 (NT) 上的tanfloc/肝素聚电解质多层膜。通过扫描电子显微镜 (SEM)、X 射线光电子能谱 (XPS) 和水接触角测量对表面进行表征。为了评估表面的血液相容性，分析了纤维蛋白原吸附、因子 XII 活化以及血小板粘附和活化。研究了对革兰氏阴性铜绿假单胞菌的抗菌活性和革兰氏阳性金黄色葡萄球菌。使用荧光显微镜和扫描电镜分析细菌粘附和形态以及生物膜形成。纤维蛋白原吸附、因子 XII 活化以及血小板粘附和活化的显着降低证明了表面的抗血栓形成特性。在孵育 24 小时后，用tanfloc/肝素修饰NT 还可降低铜绿假单胞菌和金黄色葡萄球菌的粘附和增殖，且不会形成生物膜。具有tanfloc/肝素聚电解质多层膜的改性NT 表面是一种很有前途的生物材料，可用于植入物表面，因为它们具有增强的血液生物相容性和抗菌性能。