Increasing field strength versus advanced isotope labeling for NMR based fluxomics,Magnetic Resonance in Chemistry

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Increasing field strength versus advanced isotope labeling for NMR based fluxomics
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2020-01-16 , DOI: 10.1002/mrc.4988
Mickael Dinclaux ₁ , Edern Cahoreau ₁ , Pierre Millard ₁ , Fabien Létisse ₁ , Guy Lippens ₁

Affiliation

Nuclear magnetic resonance (NMR)‐based fluxomics seeks to measure the incorporation of isotope labels in selected metabolites to follow kinetically the synthesis of the latter. It can however equally be used to understand the biosynthetic origin of the same metabolites. We investigate here different NMR approaches to optimize such experiments in terms of resolution and time requirement. Using the isoleucine biosynthesis as an example, we explore the use of different field strengths ranging from 500 MHz to 1.1 GHz. Because of the different field dependence of chemical shift and heteronuclear J couplings, the spectra change at different field strengths. We equally explore the approach to silence the leucine/valine methyl signals through the use of a suitable deuterated precursor, thereby allowing selective observation of the Ile 13C labeling pattern. Combining both approaches, we arrive at an efficient procedure for the NMR‐based exploration of Ile biosynthesis.

中文翻译：

增强场强与基于 NMR 的通量组学的高级同位素标记

基于核磁共振 (NMR) 的通量组学旨在测量同位素标记在选定代谢物中的掺入，以在动力学上跟踪后者的合成。然而，它同样可以用于了解相同代谢物的生物合成来源。我们在这里研究了不同的 NMR 方法，以在分辨率和时间要求方面优化此类实验。以异亮氨酸生物合成为例，我们探索了 500 MHz 到 1.1 GHz 范围内不同场强的使用。由于化学位移和异核 J 耦合的场依赖性不同，光谱在不同场强下发生变化。我们同样探索了通过使用合适的氘化前体使亮氨酸/缬氨酸甲基信号沉默的方法，从而允许选择性观察 Ile 13C 标记模式。

更新日期：2020-01-16

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