Current study demonstrates the immunogenic role of biopolymer coated green synthesized copper oxide nanoparticles by the induction of cellular immunity through the activation immune cells. Alongside humoral immunity response was triggered by the surface coated NPs through IgG response which indicate the adjuvanic role of the nano conjugate. Th1 (Type 1 and Type 2 helper T cells) and Th2 cells were activated after the treatment with nano conjugate and act as an immunostimulant which would inhibit the proliferation of breast cancer (MCF-7) and cervical cancer (HeLa) cells in in vitro. Solid tumor induced by 4 T1 cells were also inhibited in in vivo Balb/C mice model. Secretion of pro-inflammatory cytokines and the increase in CD + 4 populations indicate the activation of immune cells in the current study. Immunotherapy by the help of metal nano conjugate can be an effective tool to eradicate the cancer cells from the system.

中文翻译：

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壳聚糖偶联绿色氧化铜纳米粒子在肿瘤免疫治疗中的免疫刺激作用

目前的研究证明了生物聚合物包覆的绿色合成氧化铜纳米粒子通过激活免疫细胞诱导细胞免疫的免疫原性作用。除了体液免疫反应由表面包被的纳米粒子通过 IgG 反应触发，这表明纳米缀合物的佐剂作用。Th1（1 型和 2 型辅助 T 细胞）和 Th2 细胞在用纳米偶联物处理后被激活，并作为免疫刺激剂在体外抑制乳腺癌 (MCF-7) 和宫颈癌 (HeLa) 细胞的增殖. 在体内 Balb/C 小鼠模型中，4 个 T1 细胞诱导的实体瘤也受到抑制。促炎细胞因子的分泌和 CD + 4 群体的增加表明当前研究中免疫细胞的激活。