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Revealing mitogenome-wide DNA methylation and RNA editing of three Ascomycotina fungi using SMRT sequencing
Mitochondrion ( IF 3.9 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.mito.2020.01.003
Chaoxia Wang 1 , Jianhua Feng 2 , Yujiao Chen 3 , Dongmei Li 2 , Li Liu 3 , Yuqian Wu 4 , Shujun Zhang 5 , Simiao Du 6 , Yaozhou Zhang 7
Affiliation  

Beauveria bassiana, Cordyceps militaris and Ophiocordyceps sinensis (Ascomycotina) are traditional Chinese medicines. Here, mitogenomes of these three Ascomycotina fungi were sequenced and de-novo assembled using single-molecule real-time sequencing. The results showed that their complete mitogenomes were 31,258, 31,854 and 157,584 bp, respectively, with sequencing depth approximately 278,760×, 326,283× and 69,385×. Types of repeat sequences were mainly (AA)n, (AAT)n, (TA)n and (TATT)n. DNA methylation motifs were revealed in DNA modifications of these three fungi. We discovered new models of RNA editing through analysis of transcriptomes from B. bassiana and C. militaris. These data lay a solid foundation for further genetic and biological studies about these three fungi, especially for elucidating the mitogenome evolution and exploring the regulatory mechanism of adapting environment.

中文翻译:

使用 SMRT 测序揭示三种子囊菌属真菌的全线粒体 DNA 甲基化和 RNA 编辑

白僵菌、蛹虫草和冬虫夏草(子囊菌)是中药。在这里,使用单分子实时测序对这三种子囊菌属真菌的丝裂基因组进行测序和从头组装。结果表明,它们的完整有丝分裂基因组分别为31,258、31,854和157,584 bp,测序深度约为278,760×、326,283×和69,385×。重复序列的类型主要是(AA)n、(AAT)n、(TA)n和(TATT)n。在这三种真菌的 DNA 修饰中揭示了 DNA 甲基化基序。我们通过分析球虫和蛹虫草的转录组发现了新的 RNA 编辑模型。这些数据为进一步对这三种真菌进行遗传和生物学研究奠定了坚实的基础,
更新日期:2020-03-01
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