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SERS Platform for Dengue Diagnosis from Clinical Samples Employing a Hand Held Raman Spectrometer.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.analchem.9b04129 S K Gahlaut 1 , D Savargaonkar 2 , C Sharan 1 , Sarjana Yadav 1 , P Mishra 3 , J P Singh 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.analchem.9b04129 S K Gahlaut 1 , D Savargaonkar 2 , C Sharan 1 , Sarjana Yadav 1 , P Mishra 3 , J P Singh 1
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Dengue is a serious global health concern especially in tropical and subtropical countries. About 2.5 billion of the world's population is at risk for dengue infection. Early diagnosis is the key to prevent the deterioration of health of the patient to severe illness. Laboratory diagnosis of dengue is essential for providing appropriate supportive treatment to dengue patients with febrile illness, which is difficult to diagnose clinically. Here, we demonstrate surface enhanced Raman scattering (SERS) based diagnosis of dengue virus in clinical blood samples collected from total of 102 subjects. All of the samples were well characterized by conventional NS1 antigen and IgM antibody ELISA kits. The silver nanorods array fabricated by glancing angle deposition technique were employed as SERS substrates. A small amount of patient blood serum (5 μL) was taken for analysis and the report was prepared within a minute. SERS spectra of pure NS1 protein as well as spiked in serum was also recorded separately. Principal component analysis (PCA) was employed as the statistical tool to differentiate dengue positive, dengue negative, and healthy subjects on the basis of their respective SERS spectra. This method provides a sensitive, rapid, and field deployable diagnosis of dengue at the early stage (within 5 days of the onset of symptoms).
中文翻译：
使用手持拉曼光谱仪从临床样品中进行登革热诊断的SERS平台。
登革热是一个严重的全球性健康问题，尤其是在热带和亚热带国家。全球约有25亿人口面临登革热感染的风险。早期诊断是防止严重疾病患者健康恶化的关键。登革热的实验室诊断对于为高热疾病的登革热患者提供适当的支持治疗至关重要，这在临床上很难诊断。在这里，我们展示了基于表面增强拉曼散射（SERS）的登革热病毒诊断方法，该方法可从总共102名受试者收集的临床血液样本中进行。所有样品均通过常规NS1抗原和IgM抗体ELISA试剂盒很好地表征。通过掠角沉积技术制备的银纳米棒阵列被用作SERS衬底。取少量患者血清（5μL）进行分析，并在一分钟内准备好报告。还单独记录了纯NS1蛋白以及掺入血清的SERS光谱。主成分分析（PCA）被用作统计工具，以根据各自的SERS光谱区分登革热阳性，登革热阴性和健康受试者。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。和健康受试者根据各自的SERS光谱。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。和健康受试者根据各自的SERS光谱。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。
更新日期：2020-01-17
中文翻译：
使用手持拉曼光谱仪从临床样品中进行登革热诊断的SERS平台。
登革热是一个严重的全球性健康问题，尤其是在热带和亚热带国家。全球约有25亿人口面临登革热感染的风险。早期诊断是防止严重疾病患者健康恶化的关键。登革热的实验室诊断对于为高热疾病的登革热患者提供适当的支持治疗至关重要，这在临床上很难诊断。在这里，我们展示了基于表面增强拉曼散射（SERS）的登革热病毒诊断方法，该方法可从总共102名受试者收集的临床血液样本中进行。所有样品均通过常规NS1抗原和IgM抗体ELISA试剂盒很好地表征。通过掠角沉积技术制备的银纳米棒阵列被用作SERS衬底。取少量患者血清（5μL）进行分析，并在一分钟内准备好报告。还单独记录了纯NS1蛋白以及掺入血清的SERS光谱。主成分分析（PCA）被用作统计工具，以根据各自的SERS光谱区分登革热阳性，登革热阴性和健康受试者。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。和健康受试者根据各自的SERS光谱。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。和健康受试者根据各自的SERS光谱。这种方法可以在早期（症状发作后的5天内）对登革热进行灵敏，快速且可现场部署的诊断。
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