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Proteomics and Immunology Provide Insight into the Degradation Mechanism of Historic and Artificially Aged Silk.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-14 , DOI: 10.1021/acs.analchem.9b03616 Ruru Chen 1 , Mingzhou Hu 1 , Hailing Zheng 2 , Hui Yang 1 , Lian Zhou 1 , Yang Zhou 2 , Zhiqin Peng 3 , Zhiwen Hu 3 , Bing Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-14 , DOI: 10.1021/acs.analchem.9b03616 Ruru Chen 1 , Mingzhou Hu 1 , Hailing Zheng 2 , Hui Yang 1 , Lian Zhou 1 , Yang Zhou 2 , Zhiqin Peng 3 , Zhiwen Hu 3 , Bing Wang 1
Affiliation
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The process and mechanism of silk degradation is still a bewildering mystery in the investigation and conservation of cultural relics, which rely on the development of accurate and tailored analysis technologies. Here, two advanced approaches, proteomics and immunology, were developed for determining the deterioration behavior of historic silk fabrics and artificially aged samples from the molecular to the holistic level. The surface morphology and secondary structure of silk were destroyed during degradation. Subsequently, the proteomics and immunology analysis demonstrated a new degradation model differing from previous reports. First, the amorphous region and the looser crystalline regions were destroyed together, and the macromolecular chains were broken randomly. Then, the tight β-sheet blocks in the crystalline region were exposed and deteriorated, which expedited the degradation of tight β-sheet blocks and relatively loose blocks in the crystalline domain as well as the amorphous domain, ultimately yielding small molecule polypeptides. However, the deterioration process of ancient fabrics could be accelerated by poor burial conditions, thus showing distinct destructive characteristics. Overall, the results gave us a more comprehensive and profound understanding of the degradation process of ancient silk.
中文翻译：
蛋白质组学和免疫学为有历史和人工老化的丝绸的降解机理提供了见识。
丝绸降解的过程和机理在文物的调查和保存中仍然是一个令人迷惑的谜团，它依赖于精确和量身定制的分析技术的发展。在这里，开发了两种先进的方法，蛋白质组学和免疫学，用于确定历史丝绸织物和人工老化样品从分子水平到整体水平的劣化行为。在降解过程中，蚕丝的表面形态和二级结构被破坏。随后，蛋白质组学和免疫学分析证明了一种新的降解模型，与以前的报道不同。首先，无定形区域和较疏松的晶体区域一起被破坏，大分子链随机断裂。然后，结晶区域中的紧密β-折叠嵌段被暴露并恶化，这加速了结晶域和非晶域中的紧密β-折叠嵌段和相对疏松的嵌段的降解，最终产生了小分子多肽。然而，较差的埋葬条件可能会加速古代织物的变质过程，从而表现出独特的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。因此显示出明显的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。因此显示出明显的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。
更新日期：2020-01-14
中文翻译：
蛋白质组学和免疫学为有历史和人工老化的丝绸的降解机理提供了见识。
丝绸降解的过程和机理在文物的调查和保存中仍然是一个令人迷惑的谜团，它依赖于精确和量身定制的分析技术的发展。在这里，开发了两种先进的方法，蛋白质组学和免疫学，用于确定历史丝绸织物和人工老化样品从分子水平到整体水平的劣化行为。在降解过程中，蚕丝的表面形态和二级结构被破坏。随后，蛋白质组学和免疫学分析证明了一种新的降解模型，与以前的报道不同。首先，无定形区域和较疏松的晶体区域一起被破坏，大分子链随机断裂。然后，结晶区域中的紧密β-折叠嵌段被暴露并恶化，这加速了结晶域和非晶域中的紧密β-折叠嵌段和相对疏松的嵌段的降解，最终产生了小分子多肽。然而，较差的埋葬条件可能会加速古代织物的变质过程，从而表现出独特的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。因此显示出明显的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。因此显示出明显的破坏性特征。总体而言，结果使我们对古代丝绸的降解过程有了更全面，更深刻的了解。
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