Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.jprot.2020.103634 Marta L Mendes 1 , Miriam R Fougeras 2 , Gunnar Dittmar 2
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Protein ubiquitination is a powerful post-translational modification implicated in many cellular processes. Although ubiquitination is associated with protein degradation, depending on the topology of polyubiquitin chains, protein ubiquitination is connected to non-degradative events in DNA damage response, cell cycle control, immune response, trafficking, intracellular localization, and vesicle fusion events. Recently it has been shown that ubiquitin can be conjugated to two or more different chains at the same time. These branched chains add another level of complexity to ubiquitin signaling, increasing its versatility and specificity. Mass spectrometry-based proteomics has been playing an important role in the identification of all types of ubiquitin chains and linkages. This review aims to provide an overview of ubiquitin chain topology and associated signaling pathways and discusses the MS-based proteomic methodologies used to determine such topologies.
Significance
Ubiquitination plays important roles in many cellular processes. Proteins can be monoubiquitinated or polyubiquitinated forming non-branched or branched chains in a high number of possible combinations, each associated with different cellular processes. The detection and topology of ubiquitin chains is thus of extreme importance in order to explain such processes. Advances in mass spectrometry based proteomics allowed for the discovery and topology mapping of many ubiquitin chains. This review revisits the state of the art in ubiquitin chain identification by mass spectrometry and gives an insight on the implication of such chains in many cellular processes.
中文翻译：
分析泛素信号传导和链拓扑串扰。
蛋白质泛素化是许多细胞过程中涉及的强大的翻译后修饰。尽管泛素化与蛋白质降解有关，但取决于泛素链的拓扑结构，蛋白质泛素化与DNA损伤反应，细胞周期控制，免疫反应，运输，细胞内定位和囊泡融合事件中的非降解事件有关。最近，已经表明泛素可以同时缀合到两条或更多条不同的链上。这些分支链为泛素信号传导增加了另一层次的复杂性，从而增加了其多功能性和特异性。基于质谱的蛋白质组学在鉴定所有类型的泛素链和连接中一直发挥着重要作用。
意义
泛素化在许多细胞过程中起着重要作用。蛋白质可以单泛素化或多泛素化的形式以大量可能的组合形成非支链或支链，每种组合都与不同的细胞过程有关。因此，泛素链的检测和拓扑结构对于解释此类过程至关重要。基于质谱的蛋白质组学的进步使得许多泛素链的发现和拓扑图绘制成为可能。这篇综述回顾了通过质谱法鉴定遍在蛋白链的最新技术，并对这种链在许多细胞过程中的意义提供了见解。
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