The design of undergraduate laboratory courses that provide meaningful research-based experiences enhances undergraduate curricula and prepares future graduate students for research careers. In this article, a course-based undergraduate research experience (CURE) laboratory module was designed for upper-division undergraduate biochemistry and chemistry students. The laboratory module enabled students to build upon recently published data in the literature to decipher atomistic insight for an essential protein–protein interaction in human biology through the use of biomolecular NMR spectroscopy. Students compared their results with published data with the goal of identifying specific regions of the protein–protein interaction responsible for triggering an allosteric conformational change. The laboratory module introduced students to basic and advanced laboratory techniques, including protein purification, NMR spectroscopy, and analysis of protein structure using molecular visualization software.

中文翻译：

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通过 NMR 光谱研究蛋白质-蛋白质相互作用的 CURE 生物化学实验室模块

提供有意义的基于研究的经验的本科实验室课程的设计增强了本科课程，并为未来的研究生从事研究工作做好了准备。在本文中，基于课程的本科生研究体验 (CURE) 实验室模块是为高年级本科生化学和化学学生设计的。实验室模块使学生能够在文献中最近发表的数据的基础上，通过使用生物分子核磁共振波谱来破译人类生物学中基本蛋白质-蛋白质相互作用的原子论见解。学生们将他们的结果与已发表的数据进行了比较，目的是确定负责触发变构构象变化的蛋白质-蛋白质相互作用的特定区域。