Single-Molecule Kinetics Show DNA Pyrimidine Content Strongly Affects RNA:DNA and TNA:DNA Heteroduplex Dissociation Rates.,ACS Synthetic Biology

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Single-Molecule Kinetics Show DNA Pyrimidine Content Strongly Affects RNA:DNA and TNA:DNA Heteroduplex Dissociation Rates.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-01-13 , DOI: 10.1021/acssynbio.9b00471
Hershel H Lackey ₁ , Zhe Chen ₁ , Joel M Harris ₁ , Eric M Peterson ₁ , Jennifer M Heemstra _{1,

2}

Affiliation

The heteroduplex hybridization thermodynamics of DNA with either RNA or TNA are greatly affected by DNA pyrimidine content, where increased DNA pyrimidine content leads to significantly increased duplex stability. Little is known, however, about the effect that purine or pyrimidine content has on the hybridization kinetics of these duplexes. In this work, single-molecule imaging is used to measure the hybridization kinetics of oligonucleotides having varying DNA pyrimidine content with complementary DNA, RNA, and TNA sequences. Results suggest that the change in duplex stability from DNA pyrimidine content (corresponding to purine content in the complementary TNA or RNA) is primarily due to changes in the dissociation rate, and not single-strand ordering or other structural changes that increase the association rate. Decreases in heteroduplex hybridization rates with pyrimidine content are similar for RNA and TNA, indicating that TNA behaves as a kinetic analogue for RNA.

中文翻译：

单分子动力学表明DNA嘧啶含量强烈影响RNA：DNA和TNA：DNA异源双链解离速率。

DNA与RNA或TNA的异源双链杂交热力学受到DNA嘧啶含量的极大影响，其中DNA嘧啶含量的增加导致双链体稳定性的显着提高。然而，关于嘌呤或嘧啶含量对这些双链体杂交动力学的影响知之甚少。在这项工作中，单分子成像用于测量具有不同DNA嘧啶含量的寡核苷酸与互补DNA，RNA和TNA序列的杂交动力学。结果表明，DNA嘧啶含量（对应于互补TNA或RNA中嘌呤含量）的双链体稳定性变化主要是由于解离速率的变化所致，而不是单链有序化或其他增加缔合速率的结构变化。

更新日期：2020-01-13

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