Bacteriophage P1 does not show spatial preference when infecting Escherichia coli.,Virology

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Bacteriophage P1 does not show spatial preference when infecting Escherichia coli.
Virology ( IF 2.8 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.virol.2019.12.012
Kailun Zhang ₁ , Ry Young ₁ , Lanying Zeng ₁

Affiliation

To begin its infection, a bacteriophage first needs to adsorb to cells. The adsorption site on the cell surface may influence viral DNA injection, gene expression and cell-fate development. Here, we study the early steps of the infection cycle of coliphage P1, focusing on their correlation with spatial locations at the single-cell level. By fluorescently labeling P1 virions, we found that P1 shows no spatial preference on cell surface adsorption. In addition, live-cell phage DNA imaging revealed that adsorption sites do not affect the success rate for P1 in injecting its DNA into the cell. Furthermore, the lysis-lysogeny decision of P1 does not depend on the adsorption site, based on fluorescence reporters for the lytic and lysogenic pathways. These findings highlight the different infection strategies used by the two paradigmatic coliphages differ from those found in the paradigmatic phage lambda, highlighting that different infection strategies are used by phages.

中文翻译：

噬菌体 P1 在感染大肠杆菌时不表现出空间偏好。

为了开始感染，噬菌体首先需要吸附到细胞上。细胞表面的吸附位点可能会影响病毒 DNA 注射、基因表达和细胞命运的发展。在这里，我们研究了大肠杆菌噬菌体 P1 感染周期的早期步骤，重点关注它们与单细胞水平的空间位置的相关性。通过荧光标记 P1 病毒粒子，我们发现 P1 对细胞表面吸附没有表现出空间偏好。此外，活细胞噬菌体DNA成像显示吸附位点并不影响P1将其DNA注射到细胞中的成功率。此外，P1 的裂解-溶原决定不取决于吸附位点，基于裂解和溶原途径的荧光报告基因。这些发现强调了两种范例大肠杆菌噬菌体使用的不同感染策略与范例噬菌体 lambda 中发现的不同，强调噬菌体使用不同的感染策略。

更新日期：2020-01-07

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