There has been a recent increase in research interest regarding the development of wound dressings containing bioactive compounds capable of improving outcomes for complex healing needs. In the present study, we describe the generation of bromelain immobilized eletrospun poly(ε-caprolactone) (PCL) fibers (BrPDA-PCL fibers) using the dopamine-assisted co-deposition strategy. We wanted to combine the structural advantage of electrospun fiber and the activity of bromelain and PDA to develop functional wound dressings. We found that bromelain activity could be better stabilized when via its immobilization on electrospun fibers. The resultant BrPDA-PCL fibers exhibited promising properties including optimal mechanical stability, wettability, and rates of water vapor transmission. In addition, these BrPDA-PCL fibers were biocompatible, allowing for effective cellular adhesion and proliferation. The results of zone of inhibition testing further confirmed that these fibers achieved effective antibacterial activity against Escherichia coli and Staphylococcus aureus. When used in vivo, as compared with PCL fibers or control animals the BrPDA-PCL fibers enhanced wound healing rates while reducing associated inflammation. As such, these results indicate that these biocompatible BrPDA-PCL fibers exhibit desirable physicochemical properties making them ideal for use as a wound dressing to enhance the repair of full-thickness wounds to the skin.

关于包含生物活性化合物的伤口敷料的开发，最近的研究兴趣增加了，所述生物活性化合物能够改善复杂治疗需求的结果。在本研究中，我们描述了使用多巴胺辅助共沉积策略固定菠萝蛋白酶固定的静电纺丝聚（ε-己内酯）（PCL）纤维（BrPDA-PCL纤维）的过程。我们希望结合电纺纤维的结构优势以及菠萝蛋白酶和PDA的活性来开发功能性伤口敷料。我们发现，通过将菠萝蛋白酶固定在电纺纤维上可以更好地稳定其活性。所得的BrPDA-PCL纤维表现出良好的性能，包括最佳的机械稳定性，润湿性和水蒸气透过率。此外，这些BrPDA-PCL纤维具有生物相容性，允许有效的细胞粘附和增殖。抑制区测试的结果进一步证实，这些纤维对细菌具有有效的抗菌活性。大肠杆菌和金黄色葡萄球菌。当体内使用时，与PCL纤维或对照动物相比，BrPDA-PCL纤维增强了伤口的愈合速度，同时减少了相关的炎症。因此，这些结果表明，这些生物相容性BrPDA-PCL纤维表现出理想的物理化学性质，使其非常适合用作伤口敷料，以增强对皮肤的全层伤口的修复。