Evaluation of the biological properties of silanized graphene oxide is important in the context of biomedical applications of the material. In this study, we have evaluated the toxicity, immunogenicity and other biological properties like osteogenicity of silanized graphene oxide (SiGO). Graphene oxide (GO) was silanized using a common silanizing agent namely (3-aminopropyl) triethoxysilane (APTES). Silanization was confirmed through infrared spectroscopy and elemental mapping. Post-silanization, we did not observe any significant changes in the morphology of GO. Silanization leads to an increase in the interlayer distance and disorder in the lattice. Study of in vitro toxicity of SiGO on three different cell lines namely primary human dermal fibroblast, murine embryonic fibroblast and human osteosarcoma cell lines revealed that toxicity of SiGO was significantly less than GO. We further showed that in vitro immune activation of macrophage was less in the case of SiGO in comparison to GO. Profiling of osteogenic differentiation of human mesenchymal stem cell revealed that SiGO is less osteogenic than GO. Study of acute toxicity in the murine model indicated that GO was hepatotoxic at experimental concentration whereas SiGO did not show any significant toxicity. This study implied that SiGO is a better biocompatible material than GO.

在材料的生物医学应用中，硅烷化氧化石墨烯的生物学特性的评估很重要。在这项研究中，我们评估了硅烷化氧化石墨烯（SiGO）的毒性，免疫原性和其他生物学特性，例如成骨性。使用常见的硅烷化剂，即（3-氨基丙基）三乙氧基硅烷（APTES），对氧化石墨烯（GO）进行硅烷化。通过红外光谱法和元素图谱确定了硅烷化。硅烷化后，我们没有观察到GO形态发生任何显着变化。硅烷化导致层间距离的增加和晶格中的无序。SiGO对三种不同细胞系即原代人皮肤成纤维细胞的体外毒性研究 小鼠胚胎成纤维细胞和人骨肉瘤细胞系显示，SiGO的毒性显着低于GO。我们进一步表明，与GO相比，在SiGO的情况下，巨噬细胞的体外免疫激活较少。人间充质干细胞的成骨分化分析表明，SiGO的成骨性低于GO。在鼠模型中对急性毒性的研究表明，GO在实验浓度下具有肝毒性，而SiGO没有显示任何明显的毒性。这项研究表明，SiGO比GO具有更好的生物相容性。在鼠模型中对急性毒性的研究表明，GO在实验浓度下具有肝毒性，而SiGO没有显示任何明显的毒性。这项研究表明，SiGO比GO具有更好的生物相容性。在鼠模型中对急性毒性的研究表明，GO在实验浓度下具有肝毒性，而SiGO没有显示任何明显的毒性。这项研究表明，SiGO比GO具有更好的生物相容性。