Titanium alloys are commonly used in orthopedic devices due to their good corrosion resistance, high specific strength and excellent biological response. The direct contact between the implant surface and the host tissue results in notable effect of surface properties such as surface topography on the biological responses. The aim of this study is to investigate the effect of frequency of pulsed Nd-YAG laser on Ti6Al4V alloy surface topography and its influence on the improvement of biocompatibility while other laser parameters kept constant. The range of applied frequency values were selected from 1 to 20 Hz. The range of surface roughness was found between 452 nm and 3.37 μm. The untreated sample and also samples with the highest and the lowest average surface roughness parameter were subjected to the further analyses. Characterization of the samples was performed with surface roughness tester, Scanning Electron Microscopy (SEM), and Atomic Force Microscopy (AFM). The high rate of melt and solidification during the laser treatment led to the martensite formation and consequently an increase about 12–25% in hardness. Furthermore, in vitro study was carried out using MG-63 osteoblast like cells. The analyses of cell viability for 3 culture times, cell morphology and cell spreading area revealed that sample with the highest average surface roughness parameter is more biocompatible. 10 Hz frequency was found as the optimum parameter which led to the highest surface roughness and thus the biocompatibility enhancement. In conclusion, the pulsed Nd-YAG laser with an optimum value of applied frequency can be utilized as an effective technique to improve the biological characteristics.

钛合金因其良好的耐腐蚀性，高比强度和出色的生物反应性而通常用于整形外科设备。植入物表面与宿主组织之间的直接接触导致表面特性（如表面形貌）对生物学反应的显着影响。这项研究的目的是研究脉冲Nd-YAG激光的频率对Ti6Al4V合金表面形貌的影响及其对改善生物相容性的影响，同时其他激光参数保持不变。施加的频率值范围从1到20 Hz。发现表面粗糙度的范围在452nm和3.37μm之间。对未处理的样品以及具有最高和最低的平均表面粗糙度参数的样品进行进一步分析。使用表面粗糙度测试仪，扫描电子显微镜（SEM）和原子力显微镜（AFM）对样品进行表征。激光处理过程中的高熔化和凝固速率导致马氏体形成，因此硬度增加了约12–25％。此外，使用MG-63成骨细胞样细胞进行了体外研究。对3个培养时间，细胞形态和细胞扩散面积的细胞活力分析表明，具有最高平均表面粗糙度参数的样品具有更高的生物相容性。发现10 Hz频率是导致最高表面粗糙度并因此提高了生物相容性的最佳参数。结论，