This article reports the role of l-Cysteine (CYS) conjugated nanohybrid hydrogel carrier for the enhanced therapeutic delivery of the Naringenin (NRG). CYS was effectively conjugated with chitosan through the amidation reaction followed by the incorporation of phyto-synthesised zinc oxide nanoparticles (ZNPs). Dialdehyde cellulose (DAC), a green crosslinker derived from Sugarcane Bagasse (SCB) crosslinked the modified chitosan. Taguchi method optimised the NRG loading in the hydrogel carrier. The hybrid material was characterized using ¹H and ¹³C NMR, FTIR, XRD, SEM and swelling studies. The hybrid hydrogel, loaded with 86.09% of NRG was further subjected to drug release studies at varying pH and NRG loading concentrations. Maximum release of 72.78% was obtained for 1 mg/mL of initial drug concentration at pH 5. CYS conjugation with chitosan stabilized the hydrogel and enabled a sustained release of NRG drug. Kinetic modelling predicted the NRG release to follow a non-Fickian diffusion along with polymer erosion. The antimicrobial activity was studied against the Staphylococcus aureus and Trichophyton rubrum strains. Biocompatibility assay of the materials with L929 cells revealed significant cell viability. The NRG delivery using the developed nanohybrid hydrogel exhibited a two-fold increase in cytotoxicity towards A431 human skin carcinoma cells compared to NRG delivery without carrier.

本文报告的作用升半胱氨酸（CYS）共轭纳米杂化物的水凝胶载体的柚皮素（NRG）的增强的治疗递送。通过酰胺化反应，然后掺入植物合成的氧化锌纳米粒子（ZNP），CYS有效地与壳聚糖偶联。二醛纤维素（DAC）是一种衍生自甘蔗渣（SCB）的绿色交联剂，使改性的壳聚糖交联。Taguchi方法优化了NRG在水凝胶载体中的负载量。使用¹ H和¹³表征杂化材料C NMR，FTIR，XRD，SEM和溶胀研究。负载了86.09％NRG的杂化水凝胶在不同的pH和NRG负载浓度下进一步进行了药物释放研究。在pH 5时，初始药物浓度为1 mg / mL时，最大释放量为72.78％。CYS与壳聚糖的缀合稳定了水凝胶，并使NRG药物得以持续释放。动力学模型预测NRG释放将遵循非菲克扩散以及聚合物侵蚀。研究了对金黄色葡萄球菌和红毛癣菌的抗菌活性株。具有L929细胞的材料的生物相容性分析显示出明显的细胞活力。与没有载体的NRG递送相比，使用已开发的纳米杂交水凝胶的NRG递送对A431人皮肤癌细胞的细胞毒性增加了两倍。