Microalgae have been described as a sustainable feedstock for biodiesel production. In this work, Muriella decolor lipids were extracted using pressurized (compressed propane, scCO₂ and scCO₂ followed by scCO₂+ethanol) and conventional solvents (hexane, ethanol and chloroform:methanol). Total fatty acid methyl esters (FAMEs) in the unextracted biomass and solvent extracts were quantified and their profiles were determined by gas chromatography. Hexane was more selective for saponifiable lipids, whereas ethanol and chloroform:methanol resulted in higher yields of crude extracts. Among compressed fluids, propane led to both higher extraction yield and higher selectivity for saponifiable lipids, leading to 67.5% of the total FAME yield of hexane extracts. Sequential extractions using scCO₂ followed by three stages of scCO₂+EtOH improved extraction yields, reaching values (16.37 wt.% for a total FAME yield of 5.87 wt.%) higher than those obtained by conventional solvents. However, the selectivity for saponifiable matter decreased from the first extraction step.

微藻已被描述为生物柴油生产的可持续原料。在这项工作中，使用加压（压缩丙烷，scCO ₂和scCO ₂然后是scCO ₂提取）Muriella脱色脂质+乙醇）和常规溶剂（己烷，乙醇和氯仿：甲醇）。对未提取的生物质和溶剂提取物中的总脂肪酸甲酯（FAME）进行定量，并通过气相色谱法测定其分布。己烷对可皂化的脂质更具选择性，而乙醇和氯仿：甲醇可提高粗提物的收率。在压缩流体中，丙烷导致较高的提取率和对皂化脂质的较高选择性，从而导致己烷提取物的FAME总产率为67.5％。使用scCO ₂依次萃取，然后进行三个阶段的scCO ₂萃取+ EtOH改善了萃取收率，达到的值（对于FAME总收率为5.87 wt％，为16.37 wt。％）比常规溶剂获得的值高。但是，从第一提取步骤开始，对皂化物的选择性降低。