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Comparative transcriptome analysis revealed gamma-irradiation mediated disruption of floral integrator gene(s) leading to prolonged vegetative phase in Stevia rebaudiana Bertoni.
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.plaphy.2020.01.004
Gopal Singh 1 , Poonam Pal 1 , Mamta Masand 2 , Romit Seth 2 , Ashok Kumar 3 , Sanatsujat Singh 3 , Ram Kumar Sharma 1
Affiliation  

Stevia rebaudiana Bert. is getting global attention because of its ability to synthesize commercially important low/no calorie natural sweeteners (LNCSs) steviol glycosides (SGs). Considering, higher accumulation of SGs in vegetative phase followed by decrement during reproductive phase necessitate the understanding of different molecular components of floral transition to develop superior varieties/cultivars with prolonged vegetative phase in Stevia. Current comparative transcriptional analysis of low dose (5 kR) gamma-irradiated mutant genotype (SMG) with prolonged vegetative phase vis-à-vis background genotype (SBG) identified DGEs of major floral transition pathways, and expressed according to their physiological fate irrespective to SMG & SBG. Contrarily, reduced expression of floral integrator genes (FT and LEAFY) in mutant genotype suggests their involvement in prolonged vegetative phase phenotype. Likewise, GO and KEGG enrichment of photosynthesis and carbon assimilation efficiency might be associated with prolonged vegetative phase and higher accumulation of Stevioside content in mutant genotype. Furthermore, deviation of flowering related transcription factors (higher expressions except MIKS-type MADS-box SMG_PV compared to SBG_F) may possibly be correlated with low expression of floral integrator genes. Findings of current studies will facilitate the genetic manipulations and crop improvement efforts in Stevia through conventional breeding and genome editing approaches for increased SGs biosynthesis.

中文翻译:

比较转录组分析表明,伽马射线辐射介导的花整合基因破坏导致甜叶菊的营养期延长。

甜叶菊伯特。由于其能够合成商业上重要的低/无热量天然甜味剂(LNCS)甜菊糖苷(SG)而受到全球关注。考虑到,SG在营养期积累较多,随后在生殖期减少,因此有必要了解花转变的不同分子成分,以开发甜叶菊营养期延长的优良品种/栽培品种。目前对低剂量 (5 kR) γ 辐射的突变体基因型 (SMG) 与背景基因型 (SBG) 进行比较转录分析,确定了主要花转变途径的 DGE,并根据其生理命运进行表达,无论SMG 和 SBG。相反,突变基因型中花整合基因(FT 和 LEAFY)表达的减少表明它们参与了延长营养期表型。同样,光合作用和碳同化效率的 GO 和 KEGG 富集可能与突变基因型中营养期的延长和甜菊苷含量的更高积累有关。此外,开花相关转录因子的偏差(与SBG_F相比,除MIKS型MADS-box SMG_PV外的较高表达)可能与花整合基因的低表达有关。目前的研究结果将通过传统育种和基因组编辑方法促进甜菊糖的生物合成,从而促进甜叶菊的遗传操作和作物改良工作。GO和KEGG对光合作用和碳同化效率的富集可能与突变体基因型中营养期延长和甜菊苷含量更高积累有关。此外,开花相关转录因子的偏差(与SBG_F相比,除MIKS型MADS-box SMG_PV外的较高表达)可能与花整合基因的低表达有关。目前的研究结果将通过传统育种和基因组编辑方法促进甜菊糖的生物合成,从而促进甜叶菊的遗传操作和作物改良工作。GO和KEGG对光合作用和碳同化效率的富集可能与突变体基因型中营养期延长和甜菊苷含量更高积累有关。此外,开花相关转录因子的偏差(与SBG_F相比,除MIKS型MADS-box SMG_PV外的较高表达)可能与花整合基因的低表达有关。目前的研究结果将通过传统育种和基因组编辑方法促进甜菊糖的生物合成,从而促进甜叶菊的遗传操作和作物改良工作。开花相关转录因子的偏差(与SBG_F相比,除MIKS型MADS-box SMG_PV外的较高表达)可能与花整合基因的低表达有关。目前的研究结果将通过传统育种和基因组编辑方法促进甜菊糖的生物合成,从而促进甜叶菊的遗传操作和作物改良工作。开花相关转录因子的偏差(与SBG_F相比,除MIKS型MADS-box SMG_PV外的较高表达)可能与花整合基因的低表达有关。目前的研究结果将通过传统育种和基因组编辑方法促进甜菊糖的生物合成,从而促进甜叶菊的遗传操作和作物改良工作。
更新日期:2020-01-07
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