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Energy conversion processes and related gene expression in a sunflower mutant with altered salicylic acid metabolism.
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.plaphy.2020.01.005 Andrea Scartazza 1 , Marco Fambrini 2 , Lorenzo Mariotti 2 , Piero Picciarelli 2 , Claudio Pugliesi 2
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.plaphy.2020.01.005 Andrea Scartazza 1 , Marco Fambrini 2 , Lorenzo Mariotti 2 , Piero Picciarelli 2 , Claudio Pugliesi 2
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Salicylic acid (SA) is involved in several responses associated with plant development and defence against biotic and abiotic stress, but its role on photosynthetic regulation is still under debate. This work investigated energy conversion processes and related gene expression in the brachytic mutant of sunflower lingering hope (linho). This mutant was characterized by a higher ratio between the free SA form and its conjugate form SA O-β-D-glucoside (SAG) compared to wild type (WT), without significant changes in the endogenous level of abscisic acid and hydrogen peroxide. The mutant showed an inhibition of photosynthesis due to a combination of both stomatal and non-stomatal limitations, although the latter seemed to play a major role. The reduced carboxylation efficiency was associated with a down-regulation of the gene expression for both the large and small subunits of Rubisco and the Rubisco activase enzyme. Moreover, linho showed an alteration of photosystem II (PSII) functionality, with reduced PSII photochemistry, increased PSII excitation pressure and decreased thermal energy dissipation of excessive light energy. These responses were associated with a lower photosynthetic pigments concentration and a reduced expression of genes encoding for light-harvesting chlorophyll a/b binding proteins (i.e. HaLhcA), chlorophyll binding subunits of PSII proteins (i.e. HaPsbS and HaPsbX), phytoene synthase enzyme and a different expression level for genes related to PSII repair cycle, such as HaPsbA and HaPsbD. The concomitant stimulation of respiratory metabolism, suggests that linho activated a coordinate modulation of chloroplast and mitochondria activities to compensate the energy imbalance and regulate energy conversion processes.
中文翻译：
水杨酸代谢改变的向日葵突变体中的能量转化过程和相关基因表达。
水杨酸（SA）参与了与植物发育以及对生物和非生物胁迫的防御相关的多种反应，但其在光合作用调节中的作用仍在争论中。这项工作调查了向日葵挥之不去的希望（linho）的近距离突变体中的能量转换过程和相关基因表达。该突变体的特征在于，与野生型（WT）相比，游离SA形式和其缀合物形式SAO-β-D-葡萄糖苷（SAG）之间的比率更高，而脱落酸和过氧化氢的内源水平没有显着变化。该突变体由于气孔和非气孔限制的组合而显示出对光合作用的抑制，尽管后者似乎起主要作用。羧化效率的降低与Rubisco的大亚基和小亚基以及Rubisco活化酶的基因表达下调有关。此外，linho显示出光系统II（PSII）功能的变化，具有降低的PSII光化学，增加了PSII激发压力并减少了过剩光能的热能耗散。这些反应与较低的光合色素浓度和编码光捕获叶绿素a / b结合蛋白（即HaLhcA），PSII蛋白的叶绿素结合亚基（即HaPsbS和HaPsbX），八氢番茄红素合酶和与PSII修复周期相关的基因（例如HaPsbA和HaPsbD）的不同表达水平。伴随呼吸代谢的刺激，
更新日期：2020-01-07
中文翻译：
水杨酸代谢改变的向日葵突变体中的能量转化过程和相关基因表达。
水杨酸（SA）参与了与植物发育以及对生物和非生物胁迫的防御相关的多种反应，但其在光合作用调节中的作用仍在争论中。这项工作调查了向日葵挥之不去的希望（linho）的近距离突变体中的能量转换过程和相关基因表达。该突变体的特征在于，与野生型（WT）相比，游离SA形式和其缀合物形式SAO-β-D-葡萄糖苷（SAG）之间的比率更高，而脱落酸和过氧化氢的内源水平没有显着变化。该突变体由于气孔和非气孔限制的组合而显示出对光合作用的抑制，尽管后者似乎起主要作用。羧化效率的降低与Rubisco的大亚基和小亚基以及Rubisco活化酶的基因表达下调有关。此外，linho显示出光系统II（PSII）功能的变化，具有降低的PSII光化学，增加了PSII激发压力并减少了过剩光能的热能耗散。这些反应与较低的光合色素浓度和编码光捕获叶绿素a / b结合蛋白（即HaLhcA），PSII蛋白的叶绿素结合亚基（即HaPsbS和HaPsbX），八氢番茄红素合酶和与PSII修复周期相关的基因（例如HaPsbA和HaPsbD）的不同表达水平。伴随呼吸代谢的刺激，
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