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CD4+ T Follicular Helper Cells in Human Tonsils and Blood Are Clonally Convergent but Divergent from Non-Tfh CD4+ Cells.
Cell Reports ( IF 7.5 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.celrep.2019.12.016 Elena Brenna 1 , Alexey N Davydov 2 , Kristin Ladell 3 , James E McLaren 3 , Paolo Bonaiuti 4 , Maria Metsger 2 , James D Ramsden 5 , Sarah C Gilbert 6 , Teresa Lambe 6 , David A Price 7 , Suzanne L Campion 1 , Dmitriy M Chudakov 8 , Persephone Borrow 1 , Andrew J McMichael 1
Cell Reports ( IF 7.5 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.celrep.2019.12.016 Elena Brenna 1 , Alexey N Davydov 2 , Kristin Ladell 3 , James E McLaren 3 , Paolo Bonaiuti 4 , Maria Metsger 2 , James D Ramsden 5 , Sarah C Gilbert 6 , Teresa Lambe 6 , David A Price 7 , Suzanne L Campion 1 , Dmitriy M Chudakov 8 , Persephone Borrow 1 , Andrew J McMichael 1
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T follicular helper (Tfh) cells are fundamental for B cell selection and antibody maturation in germinal centers. Circulating Tfh (cTfh) cells constitute a minor proportion of the CD4+ T cells in peripheral blood, but their clonotypic relationship to Tfh populations resident in lymph nodes and the extent to which they differ from non-Tfh CD4+ cells have been unclear. Using donor-matched blood and tonsil samples, we investigate T cell receptor (TCR) sharing between tonsillar Tfh cells and peripheral Tfh and non-Tfh cell populations. TCR transcript sequencing reveals considerable clonal overlap between peripheral and tonsillar Tfh cell subsets as well as a clear distinction between Tfh and non-Tfh cells. Furthermore, influenza-specific cTfh cell clones derived from blood can be found in the repertoire of tonsillar Tfh cells. Therefore, human blood samples can be used to gain insight into the specificity of Tfh responses occurring in lymphoid tissues, provided that cTfh subsets are studied.
中文翻译：
人类扁桃体和血液中的 CD4+ T 滤泡辅助细胞与非 Tfh CD4+ 细胞在克隆上趋同但不同。
T 滤泡辅助 (Tfh) 细胞是生发中心 B 细胞选择和抗体成熟的基础。循环 Tfh (cTfh) 细胞占外周血 CD4+ T 细胞的一小部分，但它们与淋巴结中 Tfh 群的克隆型关系以及它们与非 Tfh CD4+ 细胞的差异程度尚不清楚。使用供体匹配的血液和扁桃体样本，我们研究了扁桃体 Tfh 细胞与外周 Tfh 和非 Tfh 细胞群之间的 T 细胞受体 (TCR) 共享。TCR 转录本测序揭示了外周和扁桃体 Tfh 细胞亚群之间的大量克隆重叠，以及 Tfh 和非 Tfh 细胞之间的明显区别。此外，可以在扁桃体 Tfh 细胞库中发现源自血液的流感特异性 cTfh 细胞克隆。所以，
更新日期：2020-01-07
中文翻译：
人类扁桃体和血液中的 CD4+ T 滤泡辅助细胞与非 Tfh CD4+ 细胞在克隆上趋同但不同。
T 滤泡辅助 (Tfh) 细胞是生发中心 B 细胞选择和抗体成熟的基础。循环 Tfh (cTfh) 细胞占外周血 CD4+ T 细胞的一小部分，但它们与淋巴结中 Tfh 群的克隆型关系以及它们与非 Tfh CD4+ 细胞的差异程度尚不清楚。使用供体匹配的血液和扁桃体样本，我们研究了扁桃体 Tfh 细胞与外周 Tfh 和非 Tfh 细胞群之间的 T 细胞受体 (TCR) 共享。TCR 转录本测序揭示了外周和扁桃体 Tfh 细胞亚群之间的大量克隆重叠，以及 Tfh 和非 Tfh 细胞之间的明显区别。此外，可以在扁桃体 Tfh 细胞库中发现源自血液的流感特异性 cTfh 细胞克隆。所以，
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