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RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance.
Cell Reports ( IF 7.5 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.celrep.2019.12.007 Zhuo Yang 1 , Mengmeng Li 1 , Qianwen Sun 1
Cell Reports ( IF 7.5 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.celrep.2019.12.007 Zhuo Yang 1 , Mengmeng Li 1 , Qianwen Sun 1
Affiliation
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Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R-loops when RNase H proteins are deficient. By screening factors that may relieve R-loop accumulation in the Arabidopsis atrnh1c mutant, we find that overexpression of the R-loop helicase RHON1 can rescue the defects of aberrantly accumulated HO-TRC-triggered R-loops co-transcriptionally. In addition, we find that RHON1 interacts with and orchestrates the transcriptional activity of plastid-encoded RNA polymerases to release the conflicts between transcription and replication. Our study illustrates that organisms employ multiple mechanisms to escape HO-TRC-triggered R-loop accumulation and thus maintain genome integrity.
中文翻译：
RHON1共转录解决R-环用于拟南芥叶绿体基因组维护。
防止转录复制正面冲突（HO-TRC）触发的R环形成对于维持细菌，植物和哺乳动物的基因组完整性至关重要。R环擦除器RNase H可以有效地松弛HO-TRC。但是，尚不清楚当RNase H蛋白缺乏时，生物如何抵抗HO-TRC触发的R环。通过筛选可能缓解拟南芥atrnh1c突变体中R环积累的因素，我们发现R环解旋酶RHON1的过表达可以挽救异常转录的HO-TRC触发的R环的共转录缺陷。此外，我们发现RHON1与质体编码的RNA聚合酶相互作用并协调其转录活性，以释放转录和复制之间的冲突。
更新日期：2020-01-07
中文翻译：
RHON1共转录解决R-环用于拟南芥叶绿体基因组维护。
防止转录复制正面冲突（HO-TRC）触发的R环形成对于维持细菌，植物和哺乳动物的基因组完整性至关重要。R环擦除器RNase H可以有效地松弛HO-TRC。但是，尚不清楚当RNase H蛋白缺乏时，生物如何抵抗HO-TRC触发的R环。通过筛选可能缓解拟南芥atrnh1c突变体中R环积累的因素，我们发现R环解旋酶RHON1的过表达可以挽救异常转录的HO-TRC触发的R环的共转录缺陷。此外，我们发现RHON1与质体编码的RNA聚合酶相互作用并协调其转录活性，以释放转录和复制之间的冲突。
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