The accurate quantification of neurotransmitter molecules is an indispensable means to reveal the physiological mechanisms of neuro movement in molecular level. However, existing detection strategies cannot fully meet practical needs, and the on‐site and in vivo detection of neurotransmitters in brain remains a great challenge. Here, we report the development of a near infrared light responsive photoelectrochemical (PEC) detection method for in vivo quantification of neurotransmitter dopamine in mouse brain. Under guidance of density function theory calculations, a combination strategy of non‐metal cation doping and defect engineering is introduced to rationally design the micro‐photoelectrodes with excellent biocompatibility and stability and implements the in vivo PEC detection of dopamine in mouse brain. It opens up a new way for the accurate in vivo detection of biomolecules and allows researchers to make novel inquiries for long‐standing questions in a new way.

中文翻译：

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合理设计小鼠体内神经递质分子的近红外响应微光电电极†

神经递质分子的准确定量是揭示分子水平上神经运动的生理机制的必不可少的手段。但是，现有的检测策略无法完全满足实际需求，并且在大脑中现场和体内检测神经递质仍然是一个巨大的挑战。在这里，我们报告了小鼠脑中神经递质多巴胺的体内定量近红外光响应光电化学（PEC）检测方法的发展。在密度泛函理论计算的指导下，引入非金属阳离子掺杂与缺陷工程相结合的策略，合理设计具有良好生物相容性和稳定性的微光电极，并在体内实现PEC检测小鼠脑中的多巴胺。它为准确地体内检测生物分子开辟了一条新途径，并允许研究人员以新的方式对长期存在的问题进行新颖的询问。