Understanding how defects in mechanotransduction affect cell-to-cell variability will add to the fundamental knowledge of human pluripotent stem cell (hPSC) culture, and may suggest new approaches for achieving a robust, reproducible, and scalable process that result in consistent product quality and yields. Here, the current state of the understanding of the fundamental mechanisms that govern the growth kinetics of hPSCs between static and dynamic cultures is reviewed, the factors causing fluctuations are identified, and culture strategies that might eliminate or minimize the occurrence of cell-to-cell variability arising from these fluctuations are discussed. The existing challenges in the development of hPSC expansion methods for enabling the transition from process development to large-scale production are addressed, a mandatory step for industrial and clinical applications of hPSCs.

中文翻译：

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生物工程考虑因素，以一种增强人多能干细胞可扩展性的培养方法。

了解机械转导中的缺陷如何影响细胞间的可变性将增加人类多能干细胞（hPSC）培养的基础知识，并可能提出实现稳健，可再现和可扩展的过程的新方法，从而产生一致的产品质量和产量。在此，回顾了对控制静态和动态培养之间hPSCs生长动力学的基本机制的了解的现状，确定了引起波动的因素，以及可能消除或最小化细胞间发生的培养策略。讨论了由这些波动引起的可变性。解决了hPSC扩展方法开发中的现有挑战，以实现从过程开发到大规模生产的过渡，