Integrons in Salmonella are critical genetic elements with antibiotic resistance genes that could be disseminated through horizontal gene transfer, imposing risks to public health. This study was undertaken to determine the structure of integrons in Salmonella isolated from flies on cattle farms and examine the transferability of the integrons through conjugation. Results showed that 2 out of 606 isolated Salmonella, 438 and 442, harbored class 1 integrons. Integron gene cassette in Salmonella 438 carried a single gene of aadA7, and Salmonella 442, drfA12-orfF-aadA2. The two integrons were transferrable through conjugation on microbiological media to a Salmonella strain isolated from fly and to E. coli C600 at efficiencies ranging from 1.47 × 10⁻⁶ to 6.29 × 10⁻⁴. However, only Salmonella 442 was able to transfer its integron to the recipient cells in/on 3 out of 8 farm samples, with conjugation efficiencies ranging from 4.26 × 10⁻¹⁰ to 1.36 × 10⁻⁸. Antibiotic resistance genes not carried by integrons, e.g. genes encoding resistance to tetracycline and chloramphenicol, were co-transferred with integron-mediated antibiotic resistance genes. The data suggests that some Salmonella isolated from flies of cattle source carry integrons which could disseminate antibiotic resistance genes through horizontal gene transfer in the farm environment.

沙门氏菌中的整合素是具有抗生素抗性基因的重要遗传元件，可以通过水平基因转移进行传播，从而对公共健康构成威胁。进行这项研究来确定从牛场果蝇中分离的沙门氏菌中的整合素的结构，并通过结合检查整合素的可转移性。结果显示，在606株分离的沙门氏菌中，有238株和442株中有2株具有1类整合素。沙门氏菌438中的整合子基因盒携带aadA7和沙门氏菌442的单个基因drfA12-orfF-aadA2。这两个整合素可通过在微生物培养基上偶联而转移至从蝇中分离到大肠杆菌C600中的沙门氏菌菌株，分离效率为1.47×10 ^-6至6.29×10 ^-4。但是，只有沙门氏菌442能够将其整合子转移到8个农场样本中的3个之中/之中的受体细胞上，其结合效率在4.26×10 ^-10至1.36×10 ^-8之间。整合素不携带的抗生素抗性基因，例如。编码对四环素和氯霉素抗性的基因与整合子介导的抗生素抗性基因共转移。数据表明有些沙门氏菌 从牛源蝇中分离出来的携带整联体，可以通过在农场环境中进行水平基因转移来传播抗生素抗性基因。