Rumen fluid has been applied to lignocellulosic biomass digest for methane production, and various feed supplements have been shown to improve ruminant digestion of lignocellulosic biomass. Therefore, we investigated the effects of sodium dodecyl sulfate (SDS) on the pretreatment of lignocellulosic biomass with rumen fluid and subsequent methane fermentation. SDS was mixed with rumen fluid at concentrations of 0.1, 0.2, 0.4, and 0.8 g/L. After SDS addition, the waste paper was pretreated with rumen fluid at 37 °C for 6 h. SDS addition decreased the number of surviving rumen ciliates after pretreatment. SDS addition increased the dissolved chemical oxygen demand during pretreatment; however, SDS addition did not increase the volatile fatty acid concentration. After pretreatment, batch methane fermentation of pretreated waste paper was performed at 35 °C for 45 days. SDS addition at 0.1 and 0.2 g/L shortened the waste paper digestion time and enhanced methane gas production compared to the control. By contrast, SDS addition at 0.4 and 0.8 g/L remarkably inhibited methane production from waste paper. These findings suggest that low concentrations of SDS can improve the efficiency of lignocellulosic biomass pretreatment with rumen fluid, and can enhance methane production from waste paper.

瘤胃液已被用于木质纤维素生物质的消化，以产生甲烷，并且已显示各种饲料补充剂可改善反刍动物对木质纤维素生物质的消化。因此，我们研究了十二烷基硫酸钠（SDS）对瘤胃液预处理木质纤维素生物质以及随后进行甲烷发酵的影响。将SDS与瘤胃液以0.1、0.2、0.4和0.8 g / L的浓度混合。添加SDS后，废纸用瘤胃液在37°C下预处理6小时。SDS的添加减少了预处理后存活的瘤胃纤毛的数量。SDS的添加增加了预处理过程中溶解化学需氧量；但是，添加SDS并不会增加挥发性脂肪酸的浓度。经过预处理 在35°C下对预处理过的废纸进行甲烷分批发酵45天。与对照相比，添加0.1和0.2 g / L的SDS可以缩短废纸的消化时间，并提高甲烷的产生量。相反，以0.4和0.8 g / L的SDS添加显着抑制了废纸产生的甲烷。这些发现表明，低浓度的SDS可以提高瘤胃液预处理木质纤维素生物质的效率，并可以提高废纸的甲烷产量。