Oils rich in medium‐ and long‐chain triacylglycerols (MLCT) serve as functional oils to help reduce body fat accumulation and weight gain. However, most of the MLCT‐rich products on the market are physical blends of medium‐ and long‐chain triacylglycerols (MCT and LCT, respectively) that are not structured triacylglycerols (TAG). In this study, an efficient pilot‐scale packed bed reactor (PBR) of immobilized lipase from Thermomyces lanuginosus (Lipozyme® TL IM, Novozymes, Bagsvaerd, Denmark) was employed for producing structured MLCT via 1,3‐specific interesterification of TAG enriched in caprylic and capric acyl groups and soybean oil (SBO). The PBR was operated under continuous recirculation mode in the absence of solvent. Optimal reaction conditions were determined to be: caprylic/capric TAG: SBO ratio (45:55 w/w), reaction temperature (75 °C) and residence time (16.0 min) on MLCT production were studied. When employing a pilot‐scale PBR (100 kg day⁻¹) under optimal conditions, a product containing 76.61 wt% MLCT was produced. Lipozyme TL IM was reused for 25 successive batch reactions (125 kg substrates) with no significant reduction in catalytic efficiency. The light yellow MLCT‐enriched product had a high level of saturated fatty acids (SFA, 82.74 wt%) in its sn‐2 position as a result of the enzyme's 1,3‐positional specificity. One‐stage molecular distillation and methanol extraction were used to remove the free fatty acids, mono‐, and diacylglycerols generated from hydrolysis. With distillation temperature of 150 °C and oil‐to‐methanol ratio of 1:3 v/v, MLCT content was further increased to 80.07 wt%. The enzymatic PBR was therefore effective in producing structured MLCT at a pilot‐scale under solvent‐free conditions.

中文翻译：

---

使用中试无溶剂填充床反应器，通过中链三酰基甘油和大豆油的酶促酯交换反应生产结构化三酰基甘油

富含中链和长链三酰基甘油（MLCT）的油可用作功能性油，有助于减少体内脂肪的积累和体重增加。但是，市场上大多数富含MLCT的产品都是中链和长链三酰基甘油（分别为MCT和LCT）的物理混合物，而不是结构化的三酰基甘油（TAG）。在这项研究中，采用了一种高效的中试规模的固定床脂肪酶（PBR），该床反应物来自嗜热丝霉菌（Lipozyme®TL IM，Novozymes，Bagsvaerd，丹麦），用于通过富含辛酸和癸酸基团和大豆油（SBO）的TAG的1,3特异性酯交换。在没有溶剂的情况下，PBR在连续再循环模式下运行。确定了最佳反应条件为：研究了MLCT生产的辛酸/癸酸TAG：SBO比（45:55 w / w），反应温度（75°C）和停留时间（16.0 min）。在最佳条件下使用中试规模的PBR（100千克日^-1）时，生产的产品含有76.61 wt％的MLCT。Lipozyme TL IM可重复用于25个连续的分批反应（125 kg底物），而催化效率没有明显降低。浅黄色富含MLCT的产品在其sn-2中具有高含量的饱和脂肪酸（SFA，82.74 wt％）由于酶的1,3位特异性而导致的位 使用一级分子蒸馏和甲醇萃取去除水解产生的游离脂肪酸，单和二酰基甘油。在150°C的蒸馏温度和1：3 v / v的油/甲醇比的情况下，MLCT含量进一步提高到80.07 wt％。因此，酶法PBR在无溶剂条件下以中试规模生产结构化MLCT有效。