BACKGROUND To study the therapeutic effect of bone marrow-derived mesenchymal stem cells (BMSC) against retinal neovascularization and to compare with anti-vascular endothelial growth factor (VEGF) therapy. METHODS Neonatal C57BL/6 mice were exposed in hyperoxygen and returned to room air to develop oxygen-induced retinopathy (OIR). Red fluorescent protein-labeled BMSC and Conbercept were intravitreally injected into OIR mice, respectively. Inhibition of neovascularization and apoptosis in OIR mice were assessed through retinal angiography, histopathology and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. RESULTS BMSC were able to migrate and integrate into the host retina, significantly inhibit retinal neovascular tufts and remodel the capillary network after injecton. Treatment with BMSC increased the retinal vascular density, decreased the number of acellular capillaries and inhibited retinal cell death. This effect was not inferior to current anti-VEGF therapy by using Conbercept. CONCLUSIONS Intravitreal injection of BMSC exerts a protective effect against retinal neovascularization and offers a therapeutic strategy for oxygen-induced retinopathy.

中文翻译：

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氧诱导视网膜病变小鼠模型对视网膜新生血管的治疗作用：骨髓间充质干细胞与康柏西普。


背景：研究骨髓间充质干细胞（BMSC）对抗视网膜新生血管的治疗效果，并与抗血管内皮生长因子（VEGF）治疗进行比较。方法 新生 C57BL/6 小鼠暴露在高氧环境中并返回室内空气，以发生氧诱导性视网膜病变 (OIR)。将红色荧光蛋白标记的BMSC和康柏西普分别注射到OIR小鼠的玻璃体内。通过视网膜血管造影、组织病理学和末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记 (TUNEL) 测定来评估 OIR 小鼠新血管形成和细胞凋亡的抑制。结果注射后BMSC能够迁移并整合到宿主视网膜中，显着抑制视网膜新生血管簇并重塑毛细血管网络。 BMSC治疗增加了视网膜血管密度，减少了无细胞毛细血管的数量并抑制了视网膜细胞死亡。这一效果并不逊于目前使用康柏西普的抗VEGF疗法。结论玻璃体内注射BMSC对视网膜新生血管具有保护作用，为氧诱导性视网膜病变提供了一种治疗策略。