BACKGROUND Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain and its derivatives are widely used to study the biology of this bacterium, however recent studies demonstrated differences in the genomes and phenotypes of derivatives from different laboratories. RESULTS Here we report the genome sequence of P. aeruginosa PAO1161 laboratory strain, a leu-, RifR, restriction-modification defective PAO1 derivative, described as the host of IncP-8 plasmid FP2, conferring the resistance to mercury. Comparison of PAO1161 genome with PAO1-UW sequence revealed lack of an inversion of a large genome segment between rRNA operons and 100 nucleotide polymorphisms, short insertions and deletions. These included a change in leuA, resulting in E108K substitution, which caused leucine auxotrophy and a mutation in rpoB, likely responsible for the rifampicin resistance. Nonsense mutations were detected in PA2735 and PA1939 encoding a DNA methyltransferase and a putative OLD family endonuclease, respectively. Analysis of revertants in these two genes showed that PA2735 is a component of a restriction-modification system, independent of PA1939. Moreover, a 12 kb RPG42 prophage and a novel 108 kb PAPI-1 like integrative conjugative element (ICE) encompassing a mercury resistance operon were identified. The ICEPae1161 was transferred to Pseudomonas putida cells, where it integrated in the genome and conferred the mercury resistance. CONCLUSIONS The high-quality P. aeruginosa PAO1161 genome sequence provides a reference for further research including e.g. investigation of horizontal gene transfer or comparative genomics. The strain was found to carry ICEPae1161, a functional PAPI-1 family integrative conjugative element, containing loci conferring mercury resistance, in the past attributed to the FP2 plasmid of IncP-8 incompatibility group. This indicates that the only known member of IncP-8 is in fact an ICE.

中文翻译：

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铜绿假单胞菌PAO1161的基因组序列，它是带有ICEPae1161整合和结合元件的PAO1衍生物。

背景技术铜绿假单胞菌是引起医院感染的原因，尤其是在患有囊性纤维化和烧伤伤口的患者中。PAO1菌株及其衍生物广泛用于研究这种细菌的生物学，但是最近的研究表明，来自不同实验室的衍生物的基因组和表型存在差异。结果在这里，我们报告了铜绿假单胞菌PAO1161实验室菌株的亮氨酸，RifR，限制性修饰缺陷型PAO1衍生物的基因组序列，该衍生物被描述为IncP-8质粒FP2的宿主，赋予了对汞的抗性。PAO1161基因组与PAO1-UW序列的比较表明，rRNA操纵子与100个核苷酸的多态性，短插入和缺失之间没有大的基因组片段倒置。其中包括leuA发生变化，导致E108K取代，引起亮氨酸营养缺陷和rpoB突变，这可能是利福平耐药的原因。在PA2735和PA1939中检测到无意义的突变，分别编码DNA甲基转移酶和假定的OLD家族核酸内切酶。对这两个基因的回复子的分析表明，PA2735是限制性修饰系统的一个组成部分，独立于PA1939。此外，还确定了一个12 kb的RPG42噬菌体和一个新型的108 kb的PAPI-1样整合性共轭元件（ICE），其中包含抗汞操纵子。ICEPae1161被转移到恶臭假单胞菌细胞中，并整合到基因组中并赋予了抗汞性。结论高质量的铜绿假单胞菌PAO1161基因组序列为进一步研究提供了参考，包括 研究水平基因转移或比较基因组学。过去发现该菌株携带ICEPae1161，ICEPae1161是一种功能性PAPI-1家族整合共轭元件，含有赋予汞抗性的基因座，这归因于IncP-8不相容基团的FP2质粒。这表明IncP-8的唯一已知成员实际上是ICE。