Anti-obesity effects of α-amylase inhibitor enriched-extract from white common beans (Phaseolus vulgaris L.) associated with the modulation of gut microbiota composition in high-fat diet-induced obese rats.,Food & Function

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Anti-obesity effects of α-amylase inhibitor enriched-extract from white common beans (Phaseolus vulgaris L.) associated with the modulation of gut microbiota composition in high-fat diet-induced obese rats.
Food & Function ( IF 5.1 ) Pub Date : 2020-02-05 , DOI: 10.1039/c9fo01813a
Zhenxing Shi ₁ , Yingying Zhu , Cong Teng , Yang Yao , Guixing Ren , Aurore Richel

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α-Amylase inhibitors (α-AI) have great potential to treat obesity. In this study, an α-AI enriched extract (α-AIE) with a specific activity of 1027.1 ± 154.2 (U per mg protein) was prepared from white common bean (Phaseolus vulgaris L.) seeds. Its anti-obesity effect and gut microbiota modulation properties were verified in high-fat diet-induced obese rats. The intake of the α-AIE significantly reduced body weight gain and improved serum lipid levels (p < 0.05). In addition, rats fed the α-AIE diet exhibited higher total short-chain fatty-acid (SCFA) concentrations (p < 0.05) in their colonic contents. β-Diversity analysis, principal component analysis and a Venn diagram showed that α-AIE administration changed the gut microbiota composition. At the phylum level, the relative abundances of Firmicutes and Proteobacteria decreased and the relative abundances of Bacteroidetes and Akkermansia increased. In addition, 89 operational taxonomic units (OTUs) significantly responding to the high-fat diet and 30 OTUs significantly responding to the α-AIE were identified. The OTUs enriched by the α-AIE were mainly assigned to putative SCFA-producing bacteria, including Bacteroides, Butyricoccus, Blautia and Eubacterium. Twenty-two OTUs were found to be significantly correlated with obesity indexes. Taken together, the present results suggest that the intake of the α-AIE attenuated obesity and modulated gut microbiota.

中文翻译：

高脂饮食诱导的肥胖大鼠中白普通豆（菜豆）的α-淀粉酶抑制剂富集提取物的抗肥胖作用与肠道菌群组成的调节有关。

α-淀粉酶抑制剂（α-AI）具有治疗肥胖症的巨大潜力。在这项研究中，从白色普通豆（菜豆）种子中制备了比活性为1027.1±154.2（U / mg蛋白）的富含α-AI的提取物（α-AIE）。在高脂饮食诱导的肥胖大鼠中证实了其抗肥胖作用和肠道微生物群调节特性。摄入α-AIE可显着降低体重增加并改善血脂水平（p <0.05）。此外，喂食α-AIE饮食的大鼠结肠内容物的总短链脂肪酸（SCFA）浓度较高（p <0.05）。β-多样性分析，主成分分析和维恩图显示α-AIE的施用改变了肠道菌群的组成。在门级，纤毛虫和变形杆菌的相对丰度下降，拟杆菌和阿克曼虫的相对丰度增加。此外，还确定了89个对高脂饮食有显着响应的操作分类单位（OTU）和30个对α-AIE有显着响应的OTU。由α-AIE富集的OTU主要分配给推定的产生SCFA的细菌，包括拟杆菌，丁球菌，Blautia和真细菌。发现22个OTU与肥胖指数显着相关。综上所述，目前的结果表明α-AIE的摄入减轻了肥胖并且调节了肠道菌群。确定了对高脂饮食有显着反应的89个操作分类单位（OTU）和对α-AIE有显着反应的30个OTU。由α-AIE富集的OTU主要分配给推定的产生SCFA的细菌，包括拟杆菌，丁球菌，Blautia和真细菌。发现22个OTU与肥胖指数显着相关。综上所述，目前的结果表明α-AIE的摄入减轻了肥胖并且调节了肠道菌群。确定了对高脂饮食有显着反应的89个操作分类单位（OTU）和对α-AIE有显着反应的30个OTU。由α-AIE富集的OTU主要分配给推定的产生SCFA的细菌，包括拟杆菌，丁球菌，Blautia和真细菌。发现22个OTU与肥胖指数显着相关。综上所述，目前的结果表明α-AIE的摄入减轻了肥胖并且调节了肠道菌群。

更新日期：2020-02-26

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