Main conclusion In Hordeum vulgare, nine differentially expressed novel miRNAs were induced by colchicine. Five novel miRNA in colchicine solution showed the opposite expression patterns as those in water. Abstract Colchicine is a commonly used agent for plant chromosome set doubling. MicroRNA-mediated responses to colchicine treatment in plants have not been characterized. Here, we characterized new microRNAs induced by colchicine treatment in Hordeum vulgare using high-throughput sequencing. Our results showed that 39 differentially expressed miRNAs were affected by water treatment, including 34 novel miRNAs and 5 known miRNAs; 42 miRNAs, including 37 novel miRNAs and 5 known miRNAs, were synergistically affected by colchicine and water, and 9 differentially expressed novel miRNAs were induced by colchicine. The novel_mir69, novel_mir57, novel_mir75, novel_mir38, and novel_mir56 in colchicine treatment showed the opposite expression patterns as those in water. By analyzing these 9 differentially expressed novel miRNAs and their targets, we found that novel_mir69, novel_mir56 and novel_mir25 co-target the genes involving the DNA repair pathway. Based on our results, microRNA-target regulation network under colchicine treatment was proposed, which involves actin, cell cycle regulation, cell wall synthesis, and the regulation of oxidative stress. Overall, the results demonstrated the critical role of microRNAs mediated responses to colchicine treatment in plants.

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MicroRNA 介导的对大麦秋水仙碱处理的反应

主要结论 在大麦中，秋水仙碱诱导了九个差异表达的新型 miRNA。秋水仙碱溶液中的五种新miRNA显示出与水中相反的表达模式。摘要 秋水仙碱是植物染色体组加倍的常用药剂。尚未表征植物中对秋水仙碱处理的 MicroRNA 介导的反应。在这里，我们使用高通量测序对大麦中秋水仙碱处理诱导的新 microRNA 进行了表征。我们的研究结果表明，39 个差异表达的 miRNAs 受到水处理的影响，包括 34 个新的 miRNAs 和 5 个已知的 miRNAs；秋水仙碱和水协同影响了42个miRNA，包括37个新miRNA和5个已知miRNA，秋水仙碱诱导了9个差异表达的新miRNA。小说_mir69，小说_mir57，秋水仙碱处理中的小说_mir75、小说_mir38和小说_mir56显示出与水中相反的表达模式。通过分析这 9 个差异表达的新型 miRNA 及其靶点，我们发现 Novel_mir69、novel_mir56 和 Novel_mir25 共同靶向涉及 DNA 修复途径的基因。基于我们的研究结果，提出了秋水仙碱处理下的microRNA-靶标调控网络，该网络涉及肌动蛋白、细胞周期调控、细胞壁合成和氧化应激的调控。总体而言，结果证明了 microRNA 介导的植物对秋水仙碱处理的反应的关键作用。Novel_mir56 和 Novel_mir25 共同靶向涉及 DNA 修复途径的基因。基于我们的研究结果，提出了秋水仙碱处理下的microRNA-靶标调控网络，该网络涉及肌动蛋白、细胞周期调控、细胞壁合成和氧化应激的调控。总体而言，结果证明了 microRNA 介导的植物对秋水仙碱处理的反应的关键作用。Novel_mir56 和 Novel_mir25 共同靶向涉及 DNA 修复途径的基因。基于我们的研究结果，提出了秋水仙碱处理下的microRNA-靶标调控网络，该网络涉及肌动蛋白、细胞周期调控、细胞壁合成和氧化应激的调控。总体而言，结果证明了 microRNA 介导的植物对秋水仙碱处理的反应的关键作用。