Purpose

Vitamin D deficiency (VDD) and polymorphisms in the group-specific component (GC) gene are known to be associated in different populations. However, the effects of such genetic variants may vary across different populations. Thus, the objective of this study was to estimate the association between Vitamin D-Binding Protein (VDBP) haplotypes and VDD in mestizo postmenopausal women and Mexican Amerindian ethnic groups.

Methods

This was a cross-sectional study of 726 postmenopausal Mexican women from the Health Workers Cohort Study (HWCS) and 166 postmenopausal women from the Metabolic Analysis in an Indigenous Sample (MAIS) cohort in Mexico. GC polymorphisms (rs7045 and rs4588) were analyzed by TaqMan probes. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured by Chemiluminescent Microparticle Immuno Assay.

Results

The prevalence of VDD serum 25(OH)D < 20 ng/mL was 43.7% in mestizo women and 44.6% in indigenous women. In HWCS, the single nucleotide polymorphisms (SNPs) rs7041 and rs4588 were associated with VDD. In addition, women from the HWCS, carrying the haplotypes GC2/2 and GC1f/2 had higher odds of VDD (OR = 2.83, 95% CI 1.14, 7.02; and OR = 2.30, 95% CI 1.40, 3.78, respectively) compared to women with haplotype 1f/1 s. These associations were not statistically significant in the MAIS cohort.

Conclusions

Our results show genetic association of the analyzed SNPs and related haplotypes, on the GC gene, with VDD in mestizo Mexican postmenopausal women. Moreover, a high prevalence of VDD with high genetic variability within the country was observed. Our results support the need for national policies for preventing VDD.

中文翻译：

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墨西哥混血儿和绝经后女性中维生素D缺乏症与维生素D结合蛋白基因的常见变异之间的关联。

目的

已知维生素D缺乏症（VDD）和特定基因组（GC）基因中的多态性与不同人群有关。但是，此类遗传变异的影响可能会因不同人群而异。因此，本研究的目的是评估绝经后绝经后妇女和墨西哥美洲印第安人族裔中维生素D结合蛋白（VDBP）单倍型与VDD之间的关联。

方法

这是一项横断面研究，来自墨西哥卫生工作者队列研究（HWCS）的726名绝经后墨西哥妇女和来自墨西哥土著样本（MAIS）的代谢分析的166名绝经后妇女。通过TaqMan探针分析了GC多态性（rs7045和rs4588）。通过化学发光微粒免疫测定法测量血清25-羟基维生素D [25（OH）D]水平。

结果

VDD血清25（OH）D <20 ng / mL的患病率在混血儿妇女中为43.7％，在土著妇女中为44.6％。在HWCS中，单核苷酸多态性（SNP）rs7041和rs4588与VDD相关。此外，携带单倍型GC2 / 2和GC1f / 2的HWCS妇女的VDD几率更高（分别为OR = 2.83、95％CI 1.14、7.02； OR = 2.30、95％CI 1.40、3.78）适用于单倍型为1f / 1 s的女性。这些关联在MAIS队列中没有统计学意义。

结论

我们的结果表明，在墨西哥绝经后的混血儿中，GC基因上分析的SNP和相关单倍型与VDD的遗传关联。此外，在该国观察到VDD的高流行和高遗传变异性。我们的结果支持制定防止VDD的国家政策的需求。