Fluorimetric determination of histidine by exploiting its inhibitory effect on the oxidation of thiamine by cobalt-containing Prussian Blue nanocubes,Microchimica Acta

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Fluorimetric determination of histidine by exploiting its inhibitory effect on the oxidation of thiamine by cobalt-containing Prussian Blue nanocubes
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-01-01 , DOI: 10.1007/s00604-019-3930-7
Zhixia Yao _{1,

2} , Hanmeng Liu _{1,

2} , Yaosheng Liu _{1,

2} , Qifang Zhang ₁ , Yongxing Diao _{1,

2} , Yujing Sun _{1,

2,

3} , Zhuang Li _{1,

2,

3}

Affiliation

A fluorometric assay for histidine (His) is described. It is based on the inhibitory effect of His on nanocubes consisting of cobalt-containing Prussian Blue analog (CoFe NCbs), which have a strong oxidation effect on thiamine (THI) in the presence of NaOH. THI is nonfluorescent but the oxidized form (thiochrome; ThC) has a strong blue fluorescence, with excitation/emission maxima at 370/445 nm. His inhibits the oxidation effect of the CoFe NCbs due to the strong interaction between its imidazole side chain and the amino groups of the CoFe NCbs. This method is fast and has good sensitivity and selectivity. The lower detection limit is 14.3 nM of His, the linear range extends from 0.05 to 2.5 μM, and the relative standard deviation is calculated to be 1.5%. The method was successfully employed to quantify His in spiked serum samples. Graphical abstract Schematic representation of cobalt-containing Prussian Blue nanocubes (CoFe NCbs)-thiamine (THI)-based fluorometric assay for Histine (His). His inhibits the generation of thiochrome (ThC; the oxidized form of THI). The detection limit is 14.3 nM with the linear range of 0.05–2.5 μM.

中文翻译：

利用其对含钴普鲁士蓝纳米立方体氧化硫胺素的抑制作用荧光法测定组氨酸

描述了组氨酸 (His) 的荧光测定。它基于 His 对由含钴普鲁士蓝类似物 (CoFe NCbs) 组成的纳米立方体的抑制作用，在 NaOH 存在下，该纳米立方体对硫胺素 (THI) 具有强氧化作用。THI 是无荧光的，但氧化形式（硫色素；ThC）具有强烈的蓝色荧光，最大激发/发射波长为 370/445 nm。由于其咪唑侧链与 CoFe NCb 的氨基之间的强相互作用，His 抑制了 CoFe NCb 的氧化作用。该方法速度快，灵敏度和选择性好。His 的检测下限为 14.3 nM，线性范围从 0.05 到 2.5 μM，计算得出的相对标准偏差为 1.5%。该方法已成功用于定量加标血清样品中的 His。图形摘要基于含钴普鲁士蓝纳米立方体 (CoFe NCbs)-硫胺素 (THI) 的组氨酸 (His) 荧光测定的示意图。His 抑制硫色素（ThC；THI 的氧化形式）的生成。检测限为 14.3 nM，线性范围为 0.05–2.5 μM。

更新日期：2020-01-01

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