This study has identified single-nucleotide polymorphism (SNP) markers associated with nine yield-related traits in pigeonpea by using two backcross populations (BP) developed through interspecific crosses and evaluating them at two locations and 3 years. In both the populations, markers have shown strong segregation distortion; therefore, a quantitative trait locus (QTL) mapping mixed model was used. A total of 86 QTLs explaining 12-21% phenotypic variation were detected in BP-1. On the other hand, 107 QTLs explaining 11-29% phenotypic variation were detected in BP-2. Although most QTLs were environment and trait specific, few stable and consistent QTLs were also detected. Interestingly, 11 QTLs in BP-2 were associated with more than one trait. Among these QTLs, eight QTLs associated with days to 50% flowering and days to 75% maturity were located on CcLG07. One SNP "S7_14185076" marker in BP-2 population has been found associated with four traits, namely days to 50% flowering, days to 75% maturity, primary branches per plant and secondary branches per plant with positive additive effect. Hence, the present study has not only identified QTLs for yield-related traits, but also discovered novel alleles from wild species, which can be used for improvement of traits through genomics-assisted breeding.

中文翻译：

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通过对两个种间回交种群的测序基因分型和多重定位评估，确定了木豆产量相关性状的QTL。

这项研究通过使用通过种间杂交产生的两个回交群体（BP）并在两个位置和三年进行评估，鉴定了与木豆九个产量相关性状相关的单核苷酸多态性（SNP）标记。在这两个种群中，标记物均显示出强烈的偏析畸变。因此，使用了数量性状基因座（QTL）映射混合模型。在BP-1中共检测到86个QTL，它们解释了12-21％的表型变异。另一方面，在BP-2中检测到107个QTL，它们解释了11-29％的表型变异。尽管大多数QTL是特定于环境和性状的，但也没有检测到稳定和一致的QTL。有趣的是，BP-2中的11个QTL与一个以上的性状相关。在这些QTL中，CcLG07上有8个与开花至50％的天数和成熟至75％的天数相关的QTL。已经发现BP-2群体中的一种SNP“ S7_14185076”标记与四个性状相关，即开花至50％的天数，成熟至75％的天数，每株初级分支和每株次级分支具有正加性效应。因此，本研究不仅鉴定了与产量相关的性状的QTL，而且还发现了来自野生物种的新等位基因，可用于通过基因组学辅助育种改良性状。