Yield penalty and increased grain protein content traits associated with Aegilops ventricosa 7D introgression have been mapped for the first time, and they are physically distinct from the eyespot resistance locus Pch1. Wheat wild relatives represent an important source of genetic variation, but introgression of agronomically relevant genes, such as for disease resistance, may lead to the simultaneous introduction of genetically linked deleterious traits. Pch1 is a dominant gene, conferring resistance to eyespot and was introgressed to wheat from Aegilops ventricosa as part of a large segment of the 7DV chromosome. This introgression has been associated with a significant yield reduction and a concomitant increase in grain protein content. In this study, we evaluated both traits and their relationship to the location of the Pch1 gene. We found that both QTLs were clearly distinct from the Pch1 gene, being located on a different linkage group to Pch1. In addition, we found that the QTL for increased grain protein content was strong and consistent across field trials, whereas the yield penalty QTL was unstable and environmentally dependent. The yield and grain protein content QTLs were genetically linked and located in the same linkage group. This finding is due in part to the small size of the population, and to the restricted recombination between wheat 7D and Ae. ventricosa 7Dv chromosomes. Although recombination in this interval is rare, it does occur. A recombinant line containing Pch1 and 7D_KASP6, the marker associated with increase in grain protein content, but not Xwmc221, the marker associated with the yield penalty effect, was identified.

中文翻译：

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历史上与 Aegilops ventricosa 7DV 渐渗相关的减产在遗传和物理上与眼斑抗性基因 Pch1 不同。

首次绘制了与 Aegilops ventricosa 7D 渐渗相关的产量损失和增加的谷物蛋白含量特征，它们在物理上与眼斑抗性基因座 Pch1 不同。小麦野生近缘种是遗传变异的重要来源，但农艺相关基因的渗入，例如抗病性，可能导致同时引入遗传相关的有害性状。Pch1 是一个显性基因，赋予对眼斑病的抗性，并且作为 7DV 染色体的大部分片段的一部分渗入到来自 Aegilops ventricosa 的小麦中。这种基因渗入与产量显着降低和谷物蛋白质含量随之增加有关。在这项研究中，我们评估了这两个特征及其与 Pch1 基因位置的关系。我们发现这两个 QTL 明显不同于 Pch1 基因，位于与 Pch1 不同的连锁群上。此外，我们发现增加谷物蛋白质含量的 QTL 在田间试验中是强且一致的，而产量损失 QTL 不稳定且依赖于环境。产量和籽粒蛋白含量QTLs遗传连锁，位于同一连锁群。这一发现部分是由于种群规模小，以及小麦 7D 和 Ae 之间的重组受限。腹腔 7Dv 染色体。尽管在此间隔内重组很少见，但确实会发生。鉴定了包含 Pch1 和 7D_KASP6（与谷物蛋白含量增加相关的标志物，但不包含与产量惩罚效应相关的标志物 Xwmc221）的重组品系。