The published data indicate that the bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) shows a notably low degree of intraspecific variation, and most of the molecular diagnostic methods could differentiate among subspecies groups but lacked the resolution to detect genetic diversity within subspecies. The present study describes effective differentiation of 50 isolates of C. michiganensis subsp. sepedonicus by the PCR MP method using five restriction enzymes (ApaI, PstI, BamHI, XmaI, HindIII). By analysing the electrophoretic patterns obtained by the PCR MP method performed with the applied restriction enzymes, each of the 50 tested isolates could be identified and distinguished from the remaining isolates. The PCR MP method of bacterial strain identification can be used in epidemiological studies, particularly for excluding the isolates of C. michiganensis subsp. sepedonicus as a source of primary infection.

中文翻译：

---

PCR MP方法用于区分密歇根杆状杆菌亚种。败血症分离株

公开的数据表明该细菌为密歇根州细小杆菌。sepedonicus（Cms）的种内变异程度很低，大多数分子诊断方法可以区分亚种组，但缺乏检测亚种内遗传多样性的分辨率。本研究描述了50个密歇根州立梭菌亚种的有效分离。sepedonicus通过使用五个限制性内切酶（在PCR MP法阿帕余，的Pst我，BAM HI ，的Xma我，后肢III）。通过分析使用施加的限制性内切酶通过PCR MP方法获得的电泳图谱，可以鉴定出50个测试分离株中的每一个，并将其与其余分离株区分开。细菌菌株鉴定的PCR MP方法可用于流行病学研究，特别是用于排除密歇根梭菌亚种的分离株。败血症是原发感染的来源。