AimsThe aim of the study was to evaluate the possible role of endophytic fungi (EF) on Rosa powdery mildew (PM) resistance.MethodsThe endophytic fungal communities of two wild Rosa varieties with different PM susceptibilities were investigated through both culture-dependent and independent methods, and the antagonistic activities of endophytes against the PM pathogen were evaluated by pot experiments.ResultsThere were more OTUs recovered from the PM resistant variety than from the PM susceptible variety. Similarly, the culturable EF from the PM resistant variety (1333 isolates) was more than that from the PM susceptible variety (670 isolates). Consistent with this, the colonization rate of culturable EF of the PM resistant variety was significantly higher than that of the PM susceptible variety at each PM stage (p < 0.05). In addition, it was found that the dominant EF of both varieties were different using culture-dependent and independent methods. The α-diversity of both culturable and un-culturable EF were similar except at the early stage of PM infection. Pot experiments showed that the strain M7SB 41 (Seimatosporium sp.) from PM resistant variety significantly enhanced host plant PM resistance.ConclusionsThe results suggested that the endophytic fungal communities of two wild Rosa varieties with different PM susceptibilities were significantly different, and the PM resistant Rosa variety harbored more EF than susceptible Rosa variety, and some EF played an important role in host plant PM resistance.

中文翻译：

---

两个野生蔷薇品种的真菌内生群落和内生 Seimatosporium sp. 的作用。在增强宿主植物白粉病抗性方面

目的本研究的目的是评估内生真菌（EF）对蔷薇白粉病（PM）抗性的可能作用。方法通过培养依赖和独立方法研究了具有不同 PM 敏感性的两个野生蔷薇品种的内生真菌群落，盆栽实验评价了内生菌对PM病原体的拮抗活性。结果PM抗性品种比PM易感品种回收的OTUs多。同样，来自 PM 抗性品种（1333 个分离株）的可培养 EF 高于来自 PM 易感品种（670 个分离株）的 EF。与此一致的是，PM 抗性品种的可培养 EF 的定植率在每个 PM 阶段都显着高于 PM 易感品种（p < 0.05）。此外，使用依赖于培养和独立的方法发现两个品种的优势 EF 是不同的。除了在 PM 感染的早期阶段，可培养和不可培养 EF 的 α 多样性相似。盆栽试验表明抗PM品种的菌株M7SB 41（Seimatosporium sp.）显着增强了寄主植物对PM的抗性。品种比易感 Rosa 品种含有更多的 EF，并且一些 EF 在寄主植物 PM 抗性中起重要作用。除了在 PM 感染的早期阶段，可培养和不可培养 EF 的 α 多样性相似。盆栽试验表明抗PM品种的菌株M7SB 41（Seimatosporium sp.）显着增强了寄主植物对PM的抗性。品种比易感 Rosa 品种含有更多的 EF，并且一些 EF 在寄主植物 PM 抗性中起重要作用。除了在 PM 感染的早期阶段，可培养和不可培养 EF 的 α 多样性相似。盆栽试验表明抗PM品种的菌株M7SB 41（Seimatosporium sp.）显着增强了寄主植物对PM的抗性。品种比易感 Rosa 品种含有更多的 EF，并且一些 EF 在寄主植物 PM 抗性中起重要作用。