Temozolomide is an alkylating agent which is used in glioblastoma treatment. We aimed to investigate the effects of different concentrations of temozolomide and exposure time on U87MG glioblastoma cell expression of CXCR4, MMP2, MMP9 and VEGF. U87MG cells were cultured in different temozolomide concentrations and incubation time and the effects of temozolomide on inducing apoptosis was investigated. The levels of VEGF and CXCR4 expression were measured by RT-PCR and flowcytometry. Moreover, MMP2 and MMP9 activity and expression were assessed by ELISA and zymography. CXCR4 and VEGF expression levels decreased upon applying higher concentration of temozolomide. MMP2 and MMP-9 had lower activity in cells with longer exposure time or higher doses of temozolomide. Temozolomide induces the apoptosis in U87MG glioblastoma cells at therapeutic or higher dose. It is capable of decreasing their expression levels of VEGF and CXCR4.

中文翻译：

---

替莫唑胺对U87MG胶质母细胞瘤细胞CXCR4，MMP2，MMP9，VEGF的表达，抗增殖细胞毒性和凋亡特性的影响。

替莫唑胺是用于胶质母细胞瘤治疗的烷基化剂。我们旨在研究不同浓度的替莫唑胺和暴露时间对U87MG胶质母细胞瘤细胞CXCR4，MMP2，MMP9和VEGF表达的影响。在不同的替莫唑胺浓度和孵育时间下培养U87MG细胞，研究了替莫唑胺对细胞凋亡的诱导作用。通过RT-PCR和流式细胞术测量VEGF和CXCR4的表达水平。此外，通过ELISA和酶谱法评估MMP2和MMP9的活性和表达。应用更高浓度的替莫唑胺后，CXCR4和VEGF表达水平降低。MMP2和MMP-9在具有较长暴露时间或高剂量替莫唑胺的细胞中具有较低的活性。替莫唑胺以治疗或更高剂量诱导U87MG胶质母细胞瘤细胞凋亡。它能够降低其VEGF和CXCR4的表达水平。