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Biological reduction of aflatoxin B1 in yogurt by probiotic strains of Lactobacillus acidophilus and Lactobacillus rhamnosus.
Food Science and Biotechnology ( IF 2.4 ) Pub Date : 2019-12-23 , DOI: 10.1007/s10068-019-00722-5
Fatemeh Mosallaie 1 , Hossein Jooyandeh 1 , Mohammad Hojjati 1 , Ali Fazlara 2
Affiliation  

The present study was conducted to investigate the ability of two probiotic strains, L. acidophilus PTCC 1643 and L. rhamnosus PTCC 1637, to bind aflatoxin B1 (AFB1, 20 ng/ml) in comparison with yogurt starter cultures, at equal bacterial count (~ 109 LogCFU/ml) during a 21-day storage period at 4 °C. All assessed treatments exhibited high percentages of AFB1-binding, ranged from 64.56 to 96.58%. However, the ability of probiotic bacteria was statistically higher than yogurt starter cultures. Aflatoxin binding ability of the selected lactic acid bacteria was dependent on both time and bacteria species. The highest and the lowest percentages of AFB1-removal was observed at 11th day of cold storage by L. rhamnosus (96.58 ± 3.97%) and at the first day of storage for yogurt starter cultures (64.56 ± 5.32%), respectively. The stability of bacterial cells-AFB1 complex was remarkable, since only 0.84–26.75% of bounded AFB1 was released from bacterial cells after 3 times washing during the storage period.



中文翻译:


通过嗜酸乳杆菌和鼠李糖乳杆菌益生菌菌株对酸奶中的黄曲霉毒素 B1 进行生物还原。



本研究旨在调查两种益生菌菌株(嗜酸乳杆菌PTCC 1643 和鼠李糖乳杆菌PTCC 1637)与酸奶发酵剂培养物在相同细菌浓度下结合黄曲霉毒素 B 1 (AFB 1,20 ng/ml)的能力。 4 °C 下 21 天储存期间的计数 (~ 10 9 LogCFU/ml)。所有评估的处理均表现出高百分比的 AFB 1结合,范围为 64.56% 至 96.58%。然而,益生菌的能力在统计上高于酸奶发酵剂培养物。所选乳酸菌的黄曲霉毒素结合能力取决于时间和细菌种类。鼠李糖乳杆菌 ( L. rhamnosus)冷藏第 11 天 (96.58 ± 3.97%) 和酸奶发酵剂冷藏第一天 (64.56 ± 5.32%) 观察到 AFB 1-去除率最高和最低。细菌细胞-AFB 1复合物的稳定性非常显着,因为在储存期间洗涤3次后,只有0.84-26.75%的结合AFB 1从细菌细胞中释放出来。

更新日期:2019-12-23
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