OBJECTIVE To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior. METHODS A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF. RESULTS In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry. CLINICAL RELEVANCE PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.

中文翻译：

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富含血小板的纤维蛋白对细胞增殖、迁移、分化、炎症和破骨细胞生成的影响：体外研究的系统评价。

目的系统评估富血小板纤维蛋白（PRF）对体外细胞行为的影响。方法 使用 MEDLINE 数据库进行系统的电子搜索。考虑了使用 PRF 的体外研究，并筛选了截至 2018 年 6 月 31 日发表的文章。符合条件的研究是根据人类 PRF 的使用选择的。结果 总共有 1746 个标题与搜索词一起被识别，其中 37 个符合纳入标准并被选择用于数据提取。此外，主要在 2019 年发表的 16 项新研究也被纳入分析，产生 53 项研究。由于研究设计的异质性，无法进行荟萃分析。纳入的研究表明，PRF 增强增殖、迁移、粘附、和多种细胞类型的成骨分化以及细胞信号传导激活。此外，PRF 可减少炎症，抑制破骨细胞生成，并增加间充质细胞中各种生长因子的表达。尽管这些研究存在一些显着差异，但总体研究结果表明 PRF 对细胞增殖、迁移、粘附、分化和炎症具有积极作用，这表明再生牙科具有治疗潜力。临床相关性 PRF 作为生物活性分子的储存库，支持伤口愈合和骨再生。尽管 PRF 支持临床结果的细胞机制仍不清楚，但体外研究提供了可能的解释。本系统综述旨在更新现有关于 PRF 如何影响体外基本生理过程的研究。总体研究结果表明，PRF 诱导细胞增殖、迁移、粘附和分化，同时具有抗炎特性，进一步支持其在伤口愈合和骨再生方面的治疗潜力。