A pathogen of significance in the aquaculture sector, the Gram-negative marine bacterium Vibrio parahaemolyticus causes gastroenteritis associated with consumption of improperly prepared seafood. This bacterium can be controlled using lytic bacteriophages as an alternative to antibiotics. ϕVP-1 is a lytic phage of V. parahaemolyticus that was isolated from an aquafarm water sample with the aim of assessing its potential as a bio-control agent and determining its physicochemical properties and genomic sequence. Morphological analysis by transmission electron microscopy and phylogenetic analysis based on the large terminase subunit gene showed that this phage belongs to the family Myoviridae. It could infect multiple-drug-resistant (MDR) V. parahaemolyticus and V. alginolyticus strains of mangrove and seafood origin. With a maximum adsorption time of 30 min, ϕVP-1 has a short latent period of 10 min with burst size of 44 particles/cell. Whole-genome sequencing was done using the Illumina platform, and annotation was done using GeneMarkS and Prodigal. The 150,764bp genome with an overall G+C content of 41.84% had 203 putative protein-encoding open reading frames, one tRNA gene, and 66 predicted promoters. A number of putative DNA replication and regulation, DNA packaging and structure, and host lysis genes were identified. Comparison of the ϕVP-1 genome sequence to those of known Vibrio phages indicated little discernible DNA sequence similarity, suggesting that ϕVP-1 is a novel Vibrio phage. Sequence analysis revealed the presence of 64 potential ORFs with a T4-like genomic organization. In silico analysis suggested an obligate lytic life cycle and showed the absence of lysogeny or virulence genes. The complete sequence of ϕVP-1 was annotated and deposited in the GenBank database (accession no. MH363700). The genetic features of this novel phage suggest that it might be applicable for phage therapy against pathogenic strains of V. parahaemolyticus.

中文翻译：

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新型溶菌性肌病毒科噬菌体ϕVP-1的基因组表征增强了其抗MDR生物膜形成副溶血弧菌的适用性。

革兰氏阴性海洋细菌副溶血弧菌是在水产养殖领域具有重要意义的病原体，其引起的肠胃炎与食用不当海鲜有关。可以使用溶解性噬菌体作为抗生素的替代品来控制这种细菌。ϕVP-1是副溶血性弧菌的裂解性噬菌体，它是从水产养殖场水样品中分离的，目的在于评估其作为生物防治剂的潜力，并确定其理化性质和基因组序列。通过透射电子显微镜的形态分析和基于大末端酶亚基基因的系统发育分析表明，该噬菌体属于肌病毒科。它可以感染来自红树林和海产品的多重耐药性副溶血弧菌和溶藻弧菌。ϕVP-1的最大吸附时间为30分钟，具有10分钟的短潜伏期，破裂尺寸为44个颗粒/细胞。全基因组测序使用Illumina平台完成，注释使用GeneMarkS和Prodigal完成。150,764bp基因组的总G + C含量为41.84％，具有203个推定的蛋白质编码开放阅读框，一个tRNA基因和66个预测的启动子。鉴定了许多推定的DNA复制和调控，DNA包装和结构以及宿主裂解基因。ϕVP-1基因组序列与已知弧菌噬菌体的比较表明几乎没有可辨认的DNA序列相似性，这表明ϕVP-1是一种新型弧菌噬菌体。序列分析显示存在64个具有T4样基因组结构的潜在ORF。在计算机分析中显示了一个确定的溶解生命周期，并显示了溶源性或毒力基因的缺失。注释了ϕVP-1的完整序列，并将其保存在GenBank数据库中（登录号MH363700）。这种新型噬菌体的遗传特征表明，它可能适用于针对副溶血性弧菌的病原体的噬菌体治疗。